In Vivo Evaluation of Bone Regeneration by DNA/protamine Complex Treatment with Photothermal Stress Stimulation in an Aged Rat Calvarial Defect Model
In Vivo Evaluation of Bone Regeneration by DNA/protamine Complex Treatment with Photothermal Stress Stimulation in an Aged Rat Calvarial Defect Model
复制标题
DNA/鱼精蛋白复合物处理与光热应激刺激老年大鼠颅骨缺损模型中骨再生的体内评价
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
T. Fukushima
中科院分区：
文献类型：
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作者：
M. Kawaguchi;K. Mori;H. Kajiya;T. Ikebe;T. Fukushima
制备DNA/鱼精蛋白（D/P）复合物，并使用40周龄以上大鼠的颅骨缺损模型评价新骨形成能力。每天重复光热应力（42°C，15分钟），持续3个月，以评估热应力对骨愈合的影响。利用光热装置进行光热应力刺激，该装置由含有碳纳米管的藻酸盐凝胶和提供近红外光的照射器组成。与无热刺激的D/P复合物植入相比，光热刺激加速了D/P复合物的骨再生。在有或没有光热刺激的新形成的骨和外周骨之间的努普硬度值没有显著差异。显微硬度测定显示再生骨已充分成熟。本研究结果表明，光热应力刺激上调了热休克相关基因的表达诱导的骨再生。
SYNOPSIS DNA/protamine (D/P) complex was prepared and evaluated for new bone formation ability using a calvarial defect model in rats over 40 weeks old. Photothermal stress (42°C, 15 min) was repeated every day for up to 3 months to assess the effects of thermal stress on bone healing. Photothermal stress stimulation was carried out using a photothermal device, composed of an alginate gel containing carbon nanotubes and an irradiator supplying near-infrared light. Bone regeneration with the D/P complex was accelerated by photothermal stimulation compared with D/P complex implantation without thermal stimulation. There were no significant differences in Knoop hardness values between the newly formed bone, with or without photothermal stimulation, and peripheral bone. Microhardness measurement revealed that the regenerated bones were sufficiently mature. The results of this study suggested that photothermal stress stimulation upregulated bone regeneration induced by expression of heat shock-related
靶向的单壁碳纳米管介导的PT（IV）前药使用叶酸作为归巢装置。
影响因子：
15
作者：
Dhar, Shanta;Liu, Zhuang;Thomale, Juergen;Dai, Hongjie;Lippard, Stephen J.
通讯作者：
Lippard, Stephen J.