Endothelial cell-mediated regulation of aldosterone release from human adrenocortical cells
Endothelial cell-mediated regulation of aldosterone release from human adrenocortical cells
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DOI：
10.1016/j.mce.2006.12.011
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发表时间：
2007-02-01
影响因子：
4.1
通讯作者：
Willenberg, Holger S.
中科院分区：
文献类型：
--
作者：
Ansurudeen, Ishrath;Kopprasch, Steffi;Willenberg, Holger S.
内皮细胞在内分泌组织的发育和功能中起着重要作用，并且已经显示内皮细胞衍生因子调节牛肾上腺细胞中盐皮质激素的释放。在本研究中，我们分析了人内皮细胞在肾上腺皮质细胞（NCI-H295 R）合成和释放醛固酮中的作用。内皮细胞诱导的醛固酮释放是快速的，并持续48小时以上的长期效应。这种刺激作用受内皮细胞调节的持续时间的影响，并随着条件培养基的稀释度增加而线性下降。在分子水平上，可以观察到醛固酮合成酶和星星的mRNA转录增加。与内皮细胞因子的细胞相互作用增强了CRE的激活，以及星星和SF-1报告基因的启动子活性。总之，人内皮细胞是人醛固酮合成和释放的重要肾上腺内调节剂。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
Endothelial cells play an important role in the development and functioning of endocrine tissue and endothelial cell-derived factors have been shown to regulate mineralocorticoid release in bovine adrenal cells. In the present study, we analysed the role of human endothelial cells in the synthesis and release of aldosterone from adrenocortical cells (NCI-H295R). Endothelial cell-induced aldosterone release was rapid and lasted as a long-term effect over a period of 48 h. This stimulant effect was influenced by the duration of endothelial cell conditioning and decreased linearly with increasing dilutions of the conditioned medium. At the molecular level, an increase in the mRNA transcripts of aldosterone synthase and StAR could be observed. Cellular interaction with endothelial cell-factors enhanced the activation of CRE, and the promoter activity of both StAR and SF-1 reporter genes. In conclusion, human endothelial cells are important intra-adrenal regulators of human aldosterone synthesis and release. (c) 2006 Elsevier Ireland Ltd. All rights reserved.