Fusarium graminearum TRI14 is required for high virulence and DON production on wheat but not for DON synthesis in vitro
Fusarium graminearum TRI14 is required for high virulence and DON production on wheat but not for DON synthesis in vitro
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DOI：
10.1021/jf051441a
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发表时间：
2005-11-16
影响因子：
6.1
通讯作者：
Brown, DW
中科院分区：
文献类型：
--
作者：
Dyer, RB;Plattner, RD;Brown, DW
小麦赤霉病 (FHB) 由禾谷镰刀菌引起，是世界范围内的一个主要问题。 FHB 谷物产量下降，可能无法发芽，并且经常受到脱氧雪腐镰刀菌烯醇污染，这是一种与多种动物疾病和拒食有关的单端孢菌素真菌毒素。 FHB 每年造成数千万美元的损失，这凸显了开发改进的疾病控制和预防方法的必要性。先前的研究已确定脱氧雪腐镰刀菌烯醇生物合成是小麦的毒力因子。最近，我们发现与禾谷镰刀菌密切相关的孢子毛镰刀菌的TRI14基因并不是合成相关的单端孢霉烯族毒素T-2毒素所必需的。 TRI14 与数据库中任何先前描述的基因没有相似性。在这项研究中，我们研究了禾谷镰刀菌 TRI14 在脱氧雪腐镰刀菌烯醇合成和对小麦的毒力中可能发挥的作用。 TRI14 缺失突变体在破碎的玉米粒培养基上合成脱氧雪腐镰刀菌烯醇，并在复杂和基本琼脂培养基上表现出野生型菌落形态和生长速率。然而，对温室种植小麦的 FHB 测定表明，Fg Delta Tri14 突变体引起的疾病比野生型少 50-80%，并且不会在植物上产生可检测量的脱氧雪腐镰刀菌烯醇。我们讨论了 TRI14 在疾病过程中可能发挥的一些可能的作用。
Fusarium head blight (FHB) of wheat (Triticum aestivum L.), caused by the fungus Fusarium graminearum, is a major concern worldwide. FHB grain is reduced in yield, may fail to germinate, and is often contaminated with deoxynivalenol, a trichothecene mycotoxin linked to a variety of animal diseases and feed refusals.. Annual losses in the tens of millions of dollars due to FHB underscore the need to develop improved methods of disease control and prevention. Previous research has identified deoxynivalenol biosynthesis as a virulence factor on wheat. Recently, we found that the TRI14 gene of F sporotrichioides, closely related to F graminearum, was not required for synthesis of a related trichothecene, T-2 toxin. TRI14 does not share similarity with any previously described genes in the databases. In this study, we examined the role that F. graminearum TRI14 may play in both deoxynivalenol synthesis and in virulence on wheat. TRI14 deletion mutants synthesize deoxynivalenol on cracked maize kernel medium and exhibit wild-type colony morphology and growth rate on complex and minimal agar media. However, FHB assays on greenhouse-grown wheat indicate that Fg Delta Tri14 mutants cause 50-80% less disease than wild type and do not produce a detectable quantity of deoxynivalenol on plants. We discuss a number of possible roles that TRI14 may play in the disease process.