Effect of dietary bacteriophage supplementation on internal organs, fecal excretion, and ileal immune response in laying hens challenged by Salmonella Enteritidis
Effect of dietary bacteriophage supplementation on internal organs, fecal excretion, and ileal immune response in laying hens challenged by Salmonella Enteritidis
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DOI：
10.3382/ps/pex109
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发表时间：
2017-09-01
期刊：
影响因子：
4.4
通讯作者：
Kim, W. K.
中科院分区：
文献类型：
--
作者：
Adhikari, P. A.;Cosby, D. E.;Kim, W. K.
随着目前用不同饮食干预措施替代抗生素的研究，噬菌体（BP）因其影响特定细菌的能力而成为潜在的抗菌干预措施。一项研究旨在评估 BP 对抗肠炎沙门氏菌 (SE) 对蛋鸡内脏器官定植和回肠免疫反应的作用。母鸡经口和泄殖腔内接受 10(8) cfu/mL 萘啶酸抗性肠炎沙门氏菌 (SENAR) 细胞的攻击。将 32 只单梳白来航鸡随机分配至 4 种饮食处理：1) 未激发对照（阴性对照；T1），2）SENAR 激发对照（阳性对照；T2），3）SENAR 激发 + 0.1% BP（T3），4）SENAR 激发 + 0.2% BP（T4）。感染后 7 天 (dpi)，补充 0.2% BP 后，盲肠中 SENAR 的数量显着减少 (P < 0.05)。 SENAR各自的数量从T2和T3中的2.9 log cfu/gm减少至T4中的2.0 log cfu/gm。 T3 对盲肠 SENAR 数量的减少没有显着影响。在带有胆囊的肝脏 (LGB) 中观察到 SENAR 显着降低，从 T2 中的 0.75 降至 T4 中的 0.18 log cfu/gm。在脾脏中，与 T2 和 T3 (0.94 log cfu/gm) 相比，T4 显着降低 (P < 0.05) SENAR 至 0.56 log cfu/gm。 T3 在降低脾脏 SENAR 患病率方面没有显着效果。通过补充 0.2% BP (T4)，卵巢中的 SENAR 降至 0 log cfu/gm。与阳性对照 (1.57 log cfu/gm) 相比，T4 (0.71 log cfu/gm) 在 6 dpi 时粪便 SENAR 显着降低 (P < 0.05)。与阴性对照相比，SENAR 攻击后回肠中干扰素 (IFN)-γ、白细胞介素 (IL)-6 和 IL-10 的表达显着增加。这项研究表明，除了常用的益生元或益生菌外，BP 具有病原体特异性，可以作为减少 SE 定植并诱导蛋鸡免疫调节的饮食策略之一。
With the current researches on replacing antibiotics with different dietary interventions, bacteriophages (BP) are potential antimicrobial intervention because of their ability to affect specific bacteria. A study was conducted to evaluate the role of BP against Salmonella enterica serovar Enteritidis (SE) on SE internal organs colonization and ileum immune response in laying hens. Hens were challenged both orally and intracloacally with 10(8) cfu/mL cells of nalidixic acid resistant Salmonella Enteritidis (SENAR). Thirty-two Single Comb White Leghorns were randomly allocated to 4 dietary treatments: 1) unchallenged control (negative control; T1), 2) SENAR challenged control (positive control; T2), 3) SENAR challenged + 0.1% BP (T3), and 4) SENAR challenged + 0.2% BP (T4). The number of SENAR in the ceca was significantly reduced by 0.2% BP supplementation (P < 0.05) at 7 d post infection (dpi). The respective number of SENAR was reduced from 2.9 log cfu/gm in T2 and T3 to 2.0 log cfu/gm in T4. There was no significant effect of T3 on reduction of numbers of cecal SENAR. A significant reduction of SENAR was observed in the liver with gall bladder (LGB) from 0.75 in T2 to 0.18 log cfu/gm in T4. In the spleen, T4 significantly reduced (P < 0.05) SENAR to 0.56 log cfu/gm compared to T2 and T3 (0.94 log cfu/gm). There was no significant effect of T3 in reduction of prevalence of spleen SENAR. By supplementing 0.2% BP (T4), the SENAR in the ovary was reduced to 0 log cfu/gm. There was a significant reduction (P < 0.05) in fecal SENAR at 6 dpi by T4 (0.71 log cfu/gm) compared to the positive control (1.57 log cfu/gm). The expression of interferon (IFN)-Gamma, interleukin (IL)-6, and IL-10 was significantly increased in the ileum by SENAR challenge compared to the negative control. This study suggests that apart from commonly used prebiotics or probiotics, BP are pathogen-specific and can be used as one of the dietary strategies to reduce SE colonization and induce immune modulation in laying hens.