Cryo-EM structure of the αvβ8 integrin reveals a mechanism for stabilizing integrin extension.
Cryo-EM structure of the αvβ8 integrin reveals a mechanism for stabilizing integrin extension.
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DOI：
10.1038/s41594-018-0093-x
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发表时间：
2018-08
影响因子：
16.8
通讯作者：
Cheng Y
中科院分区：
文献类型：
--
作者：
Cormier A;Campbell MG;Ito S;Wu S;Lou J;Marks J;Baron JL;Nishimura SL;Cheng Y
Integrins are conformationally flexible cell surface receptors that survey the extracellular environment for their cognate ligands. Interactions with ligands are thought to be linked to global structural rearrangements involving transitions between bent, extended-closed and -open forms. Thus far, structural details are lacking for integrins in the extended conformation due to extensive flexibility between the headpiece and legs within this conformation. Here we present single-particle electron cryo-microscopy structures of human αvβ8 integrin in the extended-closed conformation, which has been considered to be a low-affinity intermediate. Our structures show the headpiece rotating about a flexible αv-knee, suggesting a ligand surveillance mechanism for integrins in their extended-closed form. Our model predicts that the extended conformation is mainly stabilized by an interface formed between flexible loops in the upper and lower domains of the αv-leg. Confirming these findings with the αvβ3 integrin suggests that our model of stabilizing the extended-closed conformation is generalizable to other integrins.
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影响因子：
6.7
作者：
Mor-Cohen, Ronit;Rosenberg, Nurit;Seligsohn, Uri
通讯作者：
Seligsohn, Uri
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作者：
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通讯作者：
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影响因子：
4.8
作者：
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通讯作者：
Nishimura, SL
影响因子：
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作者：
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通讯作者：
Walz T
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH