Quantitative trait locus mapping for seed artificial aging traits using an F-2:3 population and a recombinant inbred line population crossed from two highly related maize inbreds
Quantitative trait locus mapping for seed artificial aging traits using an F-2:3 population and a recombinant inbred line population crossed from two highly related maize inbreds
复制标题
使用 F-2:3 群体和由两个高度相关的玉米自交系杂交的重组自交系群体进行种子人工老化性状的数量性状基因座作图
DOI：
10.1111/pbr.12663
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发表时间：
2019
期刊：
影响因子：
2
通讯作者：
Gu Riliang
中科院分区：
文献类型：
--
作者：
Liu Yanan;Zhang Hongwei;Li Xuhui;Wang Feng;Lyle Demar;Sun Lianjun;Wang Guoying;Wang Jianhua;Li Li;Gu Riliang
种子寿命的数量性状基因座(QTL)定位是选育抗采后变质的现代品种的关键。X178和I178两个自交系在人工老化处理后种子活力差异较大，但多数农艺性状表现相近。在人工老化条件下，构建F2：3群体和重组自交系(RIL)群体，在5天后定位QTL。老化发芽率、相对老化发芽率、老化简单活力指数、老化初生根长、老化枝长和老化总长与老化发芽率、相对老化发芽率、老化简单活力指数呈正相关。共定位了13个QTL，分别位于5个染色体区域：chr.1：297mb(第1染色体297mb区)、chr.3：205mb、chr.4：240mb、chr.5：205mb和chr.7：155mb，其中2~4个QTL共定位在一个区域。在每个区域都定位了3-8个与衰老相关的QTL，证实了这些区域对控制不同玉米群体种子寿命的重要性。综上所述，本工作为进一步的QTL精细定位和耐衰老玉米的分子辅助育种奠定了基础。
Quantitative trait locus (QTL) mapping for seed longevity is essential for breeding modern cultivars with resistance to deterioration during postharvest storage. The inbred lines X178 and I178 showed large differences in seed vigour after artificial aging treatment, while they had similar performances in terms of most agronomic traits. An F2:3population and a recombinant inbred line (RIL) population were generated to map QTL after 5 days under artificial aging conditions. Positive correlations were observed among all investigated traits including the aging germination rate, relative aging germination rate, aging simple vigour index, aging primary root length, aging shoot length and aging total length. Thirteen QTL were identified to locate on five chromosome regions: Chr.1:297 Mb (chromosome 1 region 297 Mb), Chr.3:205 Mb, Chr.4:240 Mb, Chr.5:205 Mb and Chr.7:155 Mb, with 2 to 4 QTL co‐located on a region. In each region, 3–8 previously identified aging‐related QTL were located, confirming the importance of these regions for controlling seed longevity in different maize populations. Taken together, the results of this work provide a foundation for further QTL fine mapping and the molecular‐assisted breeding of aging tolerant maize.