Structural and functional analysis of troponins from scallop striated and human cardiac muscles.
Structural and functional analysis of troponins from scallop striated and human cardiac muscles.
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DOI：
10.1007/978-4-431-38453-3_15
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发表时间：
2007
影响因子：
--
通讯作者：
F. Yumoto;M. Tanokura
中科院分区：
文献类型：
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作者：
F. Yumoto;M. Tanokura
扇贝横纹肌收缩的 Ca2+ 调节是一种与肌球蛋白相关的 Ca2+ 调节机制，最早由 A. G. Szent-Györgyi 及其同事发现。 1,2 在肌球蛋白连接的 Ca2+ 调节中，Ca2+ 受体位点是肌球蛋白的必需轻链，并且已发现扇贝肌原纤维的 ATP 酶通过去除肌球蛋白的调节轻链 (RLC) 对 Ca2+ 脱敏，以响应二价阳离子螯合剂 (EDTA) 的处理。同时，还从扇贝横纹肌中分离出了肌钙蛋白和原肌球蛋白的三种成分，并研究了它们的一些生化特性。3-5 在这种肌钙蛋白相关的 Ca2+ 调节中，肌钙蛋白的所有三种成分（肌钙蛋白 C、I 和 T；TnC、TnI 和 TnT）和原肌球蛋白的同时存在对于肌动球蛋白 ATP 酶的调节是必要的。活性。6–10 Ca2+ 对 TnC 的作用最终诱导肌动球蛋白 ATP 酶活性。肌钙蛋白相关的 Ca2+ 调节也会因二价阳离子螯合剂（如 EDTA 或 CDTA）治疗后 TnC 的去除而脱敏。然后对扇贝肌原纤维中这两种类型的 Ca2+ 调节的相互关系进行了如下研究。 11 通过用二价阳离子螯合剂 CDTA 处理去除 RLC 和 TnC 来制备脱敏扇贝肌原纤维，并检查用 RLC 和/或 TnC 重建对脱敏肌原纤维 ATP 酶活性的影响。
The Ca2+-regulation of scallop striated muscle contraction, a Ca2+-regulation mechanism that is linked to myosin, was first discovered by A. G. Szent-Györgyi and his colleagues. 1,2 In myosin-linked Ca2+-regulation, the Ca2+-receptive site is the essential light chain of myosin, and the ATPase of the scallop myofibrils has been found to be desensitized to Ca2+by removal of the regulatory light chain (RLC) of myosin in response to treatment with a divalent cation chelator (EDTA). At the same time, three components of troponin and tropomyosin have also been isolated from scallop striated muscle, and several of their biochemical properties have been investigated.3–5 In this troponin-linked Ca2+-regulation, the concurrent presence of all three components of troponin (troponins C, I, and T; TnC, TnI, and TnT) and tropomyosin are necessary for the regulation of actomyosin ATPase activity.6–10 The action of Ca2+on TnC ultimately induces actomyosin ATPase activity. Troponin-linked Ca2+-regulation is also desensitized by the removal of TnC in response to treatment with divalent cation chelators such as EDTA or CDTA. The mutual relation of these two types of Ca2+-regulations in scallop myofibrils was then investigated as follows.11 Desensitized scallop myofibrils were prepared by removing both RLC and TnC by treatment with a divalent cation chelator, CDTA, and the effects of reconstitution with RLC and/or TnC on the ATPase activity of the desensitized myofibrils were examined.