Investigation of seedling-stage salinity tolerance QTLs using backcross lines derived from Oryza sativa L. Pokkali
Investigation of seedling-stage salinity tolerance QTLs using backcross lines derived from Oryza sativa L. Pokkali
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DOI：
10.1111/j.1439-0523.2010.01837.x
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发表时间：
2011-08-01
期刊：
影响因子：
2
通讯作者：
Gregorio, Glenn B.
中科院分区：
文献类型：
--
作者：
Alam, Rebjana;Rahman, M. Sazzadur;Gregorio, Glenn B.
利用耐盐Pokkali与耐盐IR29杂交而成的BC3F4水稻群体进行了Saltol QTL的验证，并与背景QTL进行了比较。来自高级回交群体的特定BC3F4后代即使没有Saltol等位基因，在苗期也具有相似的耐盐性。因此，通过筛选和利用12条水稻染色体上多个位点的标记进行基因分型，研究了其他与苗期耐盐性有关的qtl。使用Q-Gene进行单标记回归（SMR），检测到7个qtl高于3.0的阈值对数。使用IciMapping的单标记分析检测到与SMR鉴定的7个qtl相关的相同标记。采用IciMapping进行基于逐步回归（LRT-RSTEP）的似然比检验分析，发现BC3F4基因渗入系中有6个qtl较为合适，其中2个为SMR共有qtl，在Saltol中包含2个显著片段。新的非saltol qtl是进一步研究的目标，以评估它们在水稻苗期更高耐盐性育种中的作用。
A population of BC3F4 rice lines derived from a cross of salt-tolerant Pokkali and sensitive IR29 was developed for validation of the Saltol QTL and comparison of this QTL effect with background QTLs. Specific BC3F4 progenies from the advanced backcross population were found to have similar salinity tolerance at the seedling stage even without the Saltol alleles. Therefore, additional QTLs responsible for seedling-stage salinity tolerance were investigated by screening and genotyping with markers at multiple loci throughout the 12 rice chromosomes. Single marker regression (SMR) using Q-Gene detected seven QTLs above the threshold logarithm of the odds of 3.0. Single marker analysis using IciMapping detected the same markers linked to the seven QTLs identified by SMR. Likelihood ratio test-based on stepwise regression (LRT-RSTEP) analysis using IciMapping, more suitable for BC3F4 introgression lines located six QTLs, of which two were common with SMR and included two significant segments within Saltol. The new non-Saltol QTLs represent targets for further study to evaluate their usefulness in breeding for higher levels of seedling-stage salinity tolerance in rice.