Serum antibodies to cow's milk proteins in ulcerative colitis and Crohn's disease.
Serum antibodies to cow's milk proteins in ulcerative colitis and Crohn's disease.
复制标题
溃疡性结肠炎和克罗恩病中牛奶蛋白的血清抗体。
DOI：
10.1016/0016-5085(87)90145-4
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发表时间：
1987
期刊：
影响因子：
29.4
通讯作者：
Albini,B
中科院分区：
文献类型：
--
作者：
Knoflach,P;Park,BH;Cunningham,R;Weiser,MM;Albini,B
采用酶联免疫吸附试验检测了51例溃疡性结肠炎、49例克罗恩病和20例年龄匹配的对照者血清中抗酪蛋白、牛血清白蛋白、α-乳白蛋白、β-乳球蛋白A和β-乳球蛋白B的IgG、IgM和IgA同种型抗体。与对照组相比，炎症性肠病患者的免疫球蛋白G和免疫球蛋白M抗体显著升高。相比之下，未观察到针对5种蛋白质中的3种的免疫球蛋白A抗体的显著增加。乳蛋白抗体滴度的增加似乎是特异性的，而不是由于多克隆免疫球蛋白激活，因为溃疡性结肠炎和克罗恩病患者中天然存在的血型抗体没有升高。在克罗恩病中，通过血清α1-酸性糖蛋白浓度测定的疾病活动度与针对某些牛奶蛋白的免疫球蛋白G和免疫球蛋白A抗体滴度之间存在良好的相关性，但在溃疡性结肠炎中则不存在。这些发现表明，牛奶蛋白抗体的产生反映了这些抗原的特异性免疫。抗体同种型和疾病活动的相关性的研究可能会提供更好的了解免疫反应的饮食抗原及其可能的作用，炎症性肠病的发病机制。
Serum antibodies of immunoglobulin G, immunoglobulin M, and immunoglobulin A isotypes to five major proteins of cow's milk, casein, bovine serum albumin, α-lactalbumin, β-lactoglobulin A, and β-lactoglobulin B, were measured using enzymelinked immunosorbent assay in 51 patients with ulcerative colitis, 49 with Crohn's disease, and 20 age-matched controls. Immunoglobulin G and immunoglobulin M antibodies to cow's milk proteins were significantly elevated in patients with inflammatory bowel disease as compared to controls. In contrast, no significant increase in immunoglobulin A antibodies to 3 of 5 proteins was noted. The increased titers of antibodies to milk proteins seem to be specific and not due to a polyclonal immunoglobulin activation, as naturally occurring blood group antibodies were not elevated in patients with ulcerative colitis and Crohn's disease. A good correlation of disease activity, as measured by serumα1-acid glycoprotein concentrations, and immunoglobulin G and immunoglobulin A antibody titers against certain cow's milk proteins could be demonstrated in Crohn's disease, but not ulcerative colitis. These findings suggest that production of antibodies to cow's milk proteins reflects specific immunization with these antigens. The study of antibody isotypes and correlation with disease activity may provide better insight into the immune response to dietary antigens and its possible role in the pathogenesis of inflammatory bowel diseases.