Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material
Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material
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DOI：
10.1038/ncb2591
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发表时间：
2012-11-01
影响因子：
21.3
通讯作者：
Pelletier, Laurence
中科院分区：
文献类型：
--
作者：
Lawo, Steffen;Hasegan, Monica;Pelletier, Laurence
中心体是动物细胞的主要微管组织中心。它是由一对中心粒围绕着着丝点周围物质(PCM)组成的。传统上被描述为非晶态的PCM的架构尚不为人所知，尽管其复杂的组装模式暗示着存在一种功能性、层次化的结构。在这里，我们使用亚衍射成像来揭示相变材料的组织特征。相间相色谱组分采用中心粒周围离散直径的同心圆环分布。多个非重叠表位的位置图显示，周中心素(PCNT)是一个从中心粒延伸出来的拉长分子。我们发现PCM组分在有丝分裂的PCM中占据了可分离的空间域，这些空间域在没有微管核复合体的情况下保持不变，并进一步表明PCNT和CDK5RAP2参与了PCM的组织和组装。在全球范围内，这项工作突出了更高级别的PCM组织在中心体组装和功能调节中的作用。
The centrosome is the main microtubule organization centre of animal cells. It is composed of a centriole pair surrounded by pericentriolar material (PCM). Traditionally described as amorphous, the architecture of the PCM is not known, although its intricate mode of assembly alludes to the presence of a functional, hierarchical structure. Here we used subdiffraction imaging to reveal organizational features of the PCM. Interphase PCM components adopt a concentric toroidal distribution of discrete diameter around centrioles. Positional mapping of multiple non-overlapping epitopes revealed that pericentrin (PCNT) is an elongated molecule extending away from the centriole. We find that PCM components occupy separable spatial domains within mitotic PCM that are maintained in the absence of microtubule nucleation complexes and further implicate PCNT and CDK5RAP2 in the organization and assembly of PCM. Globally, this work highlights the role of higher-order PCM organization in the regulation of centrosome assembly and function.