Activin signaling limits the competence for retinal regeneration from the pigmented epithelium.
Activin signaling limits the competence for retinal regeneration from the pigmented epithelium.
复制标题
激活素信号传导限制了色素上皮视网膜再生的能力。
DOI：
10.1016/j.mod.2007.10.001
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发表时间：
2008
影响因子：
2.6
通讯作者：
Reh,ThomasA
中科院分区：
文献类型：
--
作者：
Sakami,Sanae;Etter,Paige;Reh,ThomasA
两栖动物视网膜的再生是由视网膜色素上皮细胞（RPE）向神经祖细胞的转分化启动的。类似的过程也发生在早期的鸡胚中，但RPE很快就失去了这种能力。限制RPE细胞再生神经视网膜的能力的因素尚不清楚;然而，通常参与眼睛发育的因素（即FGF和Pax 6）也涉及转分化。因此，我们测试了活化素（一种在RPE发育中显示出重要性的TGFβ家族信号传导蛋白）是否有助于RPE再生视网膜的能力的丧失。我们已经发现，激活素的加入阻断了RPE的再生，即使在细胞有能力的阶段。相反，激活素/TGFβ/nodal受体的小分子抑制剂可以延迟甚至逆转FGF刺激的神经视网膜再生中的发育限制。
Regeneration of the retina in amphibians is initiated by the transdifferentiation of the retinal pigmented epithelium (RPE) into neural progenitors. A similar process occurs in the early embryonic chick, but the RPE soon loses this ability. The factors that limit the competence of RPE cells to regenerate neural retina are not understood; however, factors normally involved in the development of the eye (i.e. FGF and Pax6) have also been implicated in transdifferentiation. Therefore, we tested whether activin, a TGFβ family signaling protein shown to be important in RPE development, contributes to the loss in competence of the RPE to regenerate retina. We have found that addition of activin blocks regeneration from the RPE, even during stages when the cells are competent. Conversely, a small molecule inhibitor of the activin/TGFβ/nodal receptors can delay, and even reverse, the developmental restriction in FGF-stimulated neural retinal regeneration.