Intestinal stromal tumors in a simian immunodeficiency virus-infected, simian retrovirus-2 negative rhesus macaque (Macaca mulatta)
Intestinal stromal tumors in a simian immunodeficiency virus-infected, simian retrovirus-2 negative rhesus macaque (Macaca mulatta)
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DOI：
10.1354/vp.42-3-391
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发表时间：
2005-05-01
影响因子：
2.4
通讯作者：
Rose, TM
中科院分区：
文献类型：
--
作者：
Bielefeldt-Ohmann, H;Barouch, DH;Rose, TM
在一只5.5岁的恒河猴（Macaca mulatta）实验感染猴免疫缺陷病毒（SIVsmE 660株）和CD 4（+）T细胞耗竭后，诊断出多灶性粘膜下间质瘤。该动物对猴逆转录病毒（SRV- 1、-2和-5）呈阴性。从肿瘤和脾组织的DNA聚合酶链反应分析显示，丰富的，腹膜后纤维瘤病疱疹病毒，卡波西肉瘤相关的疱疹病毒（人疱疹病毒-8），在肿瘤中的猕猴同源物的优先存在。这是证实了病毒潜伏性核抗原-1在大多数的纺锤体肿瘤细胞的细胞核。在脾脏和肿瘤组织中也检测到低水平的另一种猕猴疱疹病毒，恒河猴鼻状病毒。纺锤体细胞标记为阳性波形蛋白和CD 117，但为阴性的CD 31，CD 68，结蛋白，平滑肌细胞肌动蛋白。总的来说，这些结果表明与猿间质增生性疾病（MPD）或典型的胃肠道间质瘤（GIST）有关，但不是绝对相同。
Multifocal submucosal stromal tumors were diagnosed in a 5.5-year-old rhesus macaque (Macaca mulatta) experimentally infected with simian immunodeficiency virus, strain SIVsmE660, and CD4(+) T cell depletec. The animal was negative for simian retroviruses, SRV- 1, -2, and -5. Polymerase chain reaction analysis of DNA from tumor and spleen tissue revealed abundant, preferential presence of retroperitoneal fibromatosis herpesvirus, the macaque homologue of the Kaposi sarcoma-associated herpesvirus (human herpesvirus-8), in the tumors. This was corroborated by demonstration of viral latent nuclear antigen-1 in the nuclei of a majority of the spindeloid tumor cells. Low levels of an additional macaque herpesvirus, rhesus rhadinovirus, were also detected in the spleen and tumor tissues. The spindeloid cells labeled positively for vimentin and CD 117 but were negative for CD31, CD68, desmin, and smooth muscle cell actin. Collectively, these findings Suggest a relation to but not absolute identity with simian mesenchymoproliferative disorders (MPD) or typical gastrointestinal stromal tumors (GISTs).