Recognition of threosyl nucleotides by DNA and RNA polymerases
Recognition of threosyl nucleotides by DNA and RNA polymerases
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DOI：
10.1093/nar/gkg833
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发表时间：
2003-11-01
影响因子：
14.9
通讯作者：
Herdewijn, P
中科院分区：
文献类型：
--
作者：
Kempeneers, V;Vastmans, K;Herdewijn, P
α-L-苏糖核酸（TNA）是潜在的天然核酸，其可以充当RNA的进化替代物。我们确定了DNA或RNA聚合酶是否可以识别磷酸化苏糖核苷。我们发现，对于Vent（exo（-））DNA聚合酶和HIV逆转录酶，与它们的天然对应物相比，掺入tTTP的K（m）值增加，k（cat）值降低。我们的研究结果表明，TNA可能在DNA-RNA-蛋白质世界的进化中发挥了作用。因此，TNA可能是进化化学和生物学进一步研究的候选者。
alpha-L-Threose nucleic acids (TNA) are potentially natural nucleic acids that could have acted as an evolutionary alternative to RNA. We determined whether DNA or RNA polymerases could recognize phosphorylated threosyl nucleosides. We found that for both the Vent (exo(-)) DNA polymerase and HIV reverse transcriptase K(m) values were increased and k(cat) values decreased for the incorporation of tTTP in comparison to their natural counterparts. Our results suggest that TNA may have played a role in the evolution of the DNA-RNA-protein world. Thus, TNA may be a candidate for further studies in evolutionary chemistry and biology.