Ethylene-induced leaf senescence depends on age-related changes and OLD genes in Arabidopsis
Ethylene-induced leaf senescence depends on age-related changes and OLD genes in Arabidopsis
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DOI：
10.1093/jxb/eri287
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发表时间：
2005-11-01
影响因子：
6.9
通讯作者：
Dijkwei, PP
中科院分区：
文献类型：
--
作者：
Jing, HC;Schippers, JHM;Dijkwei, PP
乙烯只能诱导已达到一定年龄的叶片衰老。因此，乙烯诱导的衰老依赖于单个叶片的年龄相关变化(ARC)。在拟南芥LER-0、COL-0和Ws-0材料以及从LER-0背景分离的8个OLD(叶片开始死亡)突变体中，研究了乙烯与AGE在诱导叶片衰老中的关系。终龄恒定为24d的植株暴露在乙烯中3-16d。野生型材料对乙烯处理表现出共同的反应。增加乙烯处理3~12d，黄叶数增加。然而，16d的乙烯暴露会导致黄化量的减少。因此，根据处理的时间长短，乙烯既可以对ARC产生积极的影响，也可以对随后的叶片衰老诱导产生负面影响。旧的突变体对乙烯处理的反应发生了变化。在三重反应分析中，old1和old11对乙烯敏感，暴露12d的乙烯导致黄叶数量减少。乙烯处理16d后，其余6个突变体的叶片黄化程度均未见下降。结果表明，乙烯诱导衰老的效应至少可由8个基因修饰。
Ethylene can only induce senescence in leaves that have reached a defined age. Thus, ethylene-induced senescence depends on age-related changes (ARCs) of individual leaves. The relationship between ethylene and age in the induction of leaf senescence was tested in Arabidopsis Ler-0, Col-0, and Ws-0 accessions as well as in eight old ( onset of leaf death) mutants, isolated from the Ler-0 background. Plants with a constant final age of 24 d were exposed to ethylene for 3-16 d. The wild-type accessions showed a common response to the ethylene treatment. Increasing ethylene treatments of 3-12 d caused an increase in the number of yellow leaves. However, an ethylene exposure time of 16 d resulted in a decrease in the amount of yellowing. Thus, ethylene can both positively and negatively influence ARCs and the subsequent induction of leaf senescence, depending on the length of the treatment. The old mutants showed altered responses to the ethylene treatments. old1 and old11 were hypersensitive to ethylene in the triple response assay and a 12-d ethylene exposure resulted in a decrease in the amount of yellow leaves. The other six mutants did not show a decrease in yellow leaves with an ethylene treatment of 16 d. The results revealed that the effect of ethylene on the induction of senescence can be modified by at least eight genes.