Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components
Four highly stable eukaryotic mRNAs assemble 3' untranslated region RNA-protein complexes sharing cis and trans components
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DOI：
10.1073/pnas.94.6.2410
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发表时间：
1997-03-18
影响因子：
11.1
通讯作者：
Liebhaber, SA
中科院分区：
文献类型：
--
作者：
Holcik, M;Liebhaber, SA
在人α-珠蛋白mRNA的3‘非翻译区(3’UTR)上组装序列特异性的RNA-蛋白质复合体(α-Complex)与mRNA的稳定相关，在这里，我们定位了α-珠蛋白3‘UTR的一个有限片段，这是形成α-复合体的必要条件和充分条件。这个结合区的序列识别了另外三个高度稳定的mRNAs，它们在各自的3‘UTRs中分享了密切相关的富含嘧啶的顺式基序。每个mRNA在这个保守的区域组装一个序列特异性的核糖核蛋白复合体，这些复合体在结构上是相关的，每个复合体都含有一个39 kDa的细胞质Poly(C)结合蛋白，这些观察结果表明真核mRNAs的稳定存在一个普遍的决定因素。
Assembly of a sequence-specific RNA-protein complex on the 3' untranslated region (3'UTR) of human alpha-globin mRNA (alpha-complex) correlates with mRNA stabilization, Here we map a limited segment of the alpha-globin 3'UTR that is both necessary and sufficient for alpha-complex formation. The sequence of this binding region identifies three additional, highly stable mRNAs that share closely related, pyrimidine-rich cis-motifs in their respective 3'UTRs. Each mRNA assembles a sequence-specific ribonucleoprotein complex at this conserved region, These complexes are structurally related, and each contains a 39-kDa cytoplasmic poly(C) binding protein previously demonstrated to be essential to formation of the alpha-complex, These observations indicate the existence of a general determinant for stabilization of eukaryotic mRNAs.