From Mysterious Supernatant Entity to miRNA-150 in Antigen-Specific Exosomes: a History of Hapten-Specific T Suppressor Factor.
From Mysterious Supernatant Entity to miRNA-150 in Antigen-Specific Exosomes: a History of Hapten-Specific T Suppressor Factor.
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DOI：
10.1007/s00005-015-0331-4
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发表时间：
2015-10
影响因子：
3.2
通讯作者：
Bryniarski K
中科院分区：
文献类型：
--
作者：
Ptak W;Nazimek K;Askenase PW;Bryniarski K
在Gershon于1970年发现T抑制细胞后不久，研究表明，通过静脉注射半抗原诱导的这些淋巴细胞的一个亚群通过释放特异性抗原作用的可溶性T抑制因子（TsF）来抑制小鼠体内由效应CD4+或CD8+ T细胞介导的接触敏感反应。我们的实验表明，具有生物活性的TsF是由两种亚因子组成的复杂实体，一种是抗原特异性的，另一种是非特异性的，由不同诱导的细胞群产生。在接下来的几年里，我们发现抗原特异性亚因子是由B1a淋巴细胞产生的IgM抗体的轻链。然而，大约30年来，非特异性部分的确切性质一直是个谜。我们目前的研究表明，TsF是由T抑制细胞衍生的外泌体携带的调节性miRNA-150，由于B1a细胞产生的IgM抗体轻链的表面涂层而具有抗原特异性。本文简要总结了我们对TsF的研究，发现了调节miRNA特异性作用于抗原抑制免疫反应。
Soon after the discovery of T suppressor cells by Gershon in 1970, it was demonstrated that one subpopulation of these lymphocytes induced by i.v. hapten injection suppresses contact sensitivity response mediated by effector CD4+ or CD8+ T cells in mice through the release of soluble T suppressor factor (TsF) that acts antigen specifically. Our experiments showed that biologically active TsF is a complex entity consisting of two subfactors, one antigen specific and other non-specific, produced by differently induced populations of cells. In following years, we found that the antigen-specific subfactor is a light chain of IgM antibody that is produced by B1a lymphocytes. However, the exact nature of non-specific part remained a mystery for about 30 years. Our current studies characterized TsF as regulatory miRNA-150 carried by T suppressor cell-derived exosomes that are antigen specific due to a surface coat of IgM antibody light chains produced by B1a cells. The present communication briefly summarizes our studies on TsF that led to discovery of regulating miRNA that acts antigen specifically to suppress immune response.
接触灵敏度转移的抑制机制。分析LY2抑制细胞活性所需的I-J+分子。
影响因子：
15.3
作者：
Ptak, W;Gershon, R K;Flood, P M
通讯作者：
Flood, P M