Protective Immunity Elicited by VP1 Chimeric Antigens of Bacterial Ghosts against Hand-Foot-and-Mouth Disease Virus
Protective Immunity Elicited by VP1 Chimeric Antigens of Bacterial Ghosts against Hand-Foot-and-Mouth Disease Virus
复制标题
菌鬼VP1嵌合抗原对手足口病病毒的保护性免疫
DOI：
10.3390/vaccines8010061
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发表时间：
2020-03-01
期刊：
影响因子：
7.8
通讯作者：
Wang, Hui
中科院分区：
文献类型：
--
作者：
Gong, Saisai;Nan, Nan;Wang, Hui
本研究旨在评价两种菌蜕VP 1嵌合抗原的免疫原性和保护效果。将两种菌蜕VP 1嵌合抗原接种BALB/c小鼠，可引起明显的体液和粘膜免疫应答。嵌合体血影诱导的特异性抗体不仅保护小鼠抵抗引起手足口病的病毒，而且还保护小鼠抵抗大肠杆菌。coliO 157：H7细菌感染。与阴性对照相比，用嵌合体血影免疫保护小鼠免受两种LD 50手足口病病毒感染。此外，这种特异性免疫也能保护用菌蜕VP 1嵌合抗原免疫的孕鼠幼仔抵抗20 MLD E。大肠杆菌O 157：H7感染。综上所述，本研究的结果首次证实了细菌菌蜕的VP 1嵌合抗原是新型手足口病疫苗的候选靶点。此外，该疫苗策略还引起了针对E. coli O157：H7。
This study was designed to evaluate the immunogenicity and protective efficacy of two VP1 chimeric antigens of bacterial ghosts. Inoculation of the two VP1 chimeric antigens of bacterial ghosts into BALB/c mice markedly elicited humoral and mucosal immune responses. The specific antibodies induced by the chimeric ghosts protected mice not only against the virus that causes hand-foot-and-mouth disease but also against E. coli O157:H7 bacterial infection. In comparison with the negative control, immunization with the chimeric ghosts protected mice against two LD50 hand-foot-and-mouth disease viral infection. In addition, this specific immunity also protected the pups of pregnant mice immunized with the VP1 chimeric antigens of bacterial ghosts against 20 MLD E. coli O157:H7 infection. Taken together, the results of this study verify for the first time that the VP1 chimeric antigens of bacterial ghosts are target candidates for a new type of vaccine against hand-foot-and-mouth disease. Additionally, this vaccine strategy also elicited a stronger immune response against E. coli O157:H7.