BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis
BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis
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DOI：
10.1242/dev.02386
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发表时间：
2006-06-01
期刊：
影响因子：
4.6
通讯作者：
Evans, Todd
中科院分区：
文献类型：
--
作者：
Gupta, Sunny;Zhu, Hao;Evans, Todd
骨形态发生蛋白（bone morphogenetic protein， BMP）信号通路在原肠胚形成过程中对于腹侧中胚层的形成至关重要，这使得在发育后期确定该通路的功能成为一项挑战。我们已经建立了一种方法，通过在发育中的斑马鱼胚胎中将显性阴性BMP受体靶向Lmo2+细胞，在体细胞发生过程中特异性地破坏外侧中胚层的BMP信号。这导致造血细胞和内皮细胞的扩增，同时限制了原肾标志物pax2.1的表达域。一个组成活性受体的表达和移植实验被用来证实BMP信号在外侧中胚层限制随后的血管发育。结果表明，BMP信号通路在细胞向外侧中胚层发育后继续发挥作用，并通过限制血管命运以促进肾原细胞发育来影响随后的谱系决定。
The bone morphogenetic protein ( BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to de. ne functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.