Free-solution electrophoresis of DNA
Free-solution electrophoresis of DNA
复制标题
DOI：
10.1016/s0021-9673(97)00656-0
复制
发表时间：
1998-05-08
影响因子：
4.1
通讯作者：
Drouin, G
中科院分区：
文献类型：
--
作者：
Heller, C;Slater, GW;Drouin, G
我们报告了第一个实验证据，即双链DNA片段可以在自由溶液中分离（即，在不存在筛分基质的情况下），此时使用生物素化的核苷酸将单个或两个链霉亲和素分子连接到末端。如前所述，在更高的电场下或当两个链霉亲和素分子连接到每个DNA片段时获得更高的分辨率，分辨率还受毛细管直径和涂层的影响。(C)1998年Elsevier Science B.V.
We report the first experimental evidence that double-stranded DNA fragments can be electrophoretically separated in free-solution (i.e., in the absence of a sieving matrix) when either a single or two streptavidin molecules are attached to the end(s) using biotinylated nucleotides. As previously predicted, higher resolution is obtained at higher electric fields or when two streptavidin molecules are attached to each DNA fragment, The resolution is also affected by the diameter and coating of the capillaries. (C) 1998 Elsevier Science B.V.