Growth Rate Studies of Gracilaria verrucosa (Gigartinales, Rhodophyta)
Growth Rate Studies of Gracilaria verrucosa (Gigartinales, Rhodophyta)
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江蓠（Gigatinales，Rhodophyta）生长速率研究
DOI：
10.1515/botm.1987.30.3.223
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
I. Umezaki
中科院分区：
文献类型：
--
作者：
A. Hurtado;I. Umezaki
将从菲律宾甲米地的Bacoor采集的囊果植物建立的嫩的不分枝的多疣江蓠（Gracilaria verrucosa）四孢子体切成三部分（顶端、中部和基部），每部分10 mm用于生长速率研究。将它们在装有900 ml SWM-3的1-1锥形瓶中孵育，并保持在15 ℃或20 ℃下11 h光照：13 h黑暗和25 ℃或30 ℃下13 h光照：11 h黑暗，用冷白色40-W荧光灯照明2000-2090 lux。将用于第二个实验的接种物的四孢子体的全叶和半叶保持在25 °C 13：TTh的环境温度下。每周更换培养基。在30天的培养期后，测定菌体片段的比生长速率（），其表示为每天长度的增加。顶端节在25 °C时生长最快，中部和基部节在30 °C时生长最快。统计分析（ANOVA）表明，增长是显着不同的，在所有温度之间的段。温度对各节段的生长有显著影响。21天后，测定全叶和半叶的比生长率（），表示每天鲜重的增加。统计分析（学生的t检验）表明，自由浮动的半叶和附加的半叶之间的增长率和自由浮动的半叶和附加和自由浮动的全叶之间的差异非常显着。结果表明，尖瓣和半叶状体是一种潜在的大田栽培和水产养殖接种体。接种物的播种必须在适当的温度下开始，以促进快速生长，从而获得更高的产量。
Young unbranched tetrasporophytes of Gracilaria verrucosa established from cystocarpic plants collected from Bacoor, Cavite, Philippines were cut into three parts (apical, mid and basal) each of 10 mm for growth rate studies. They were incubated in 1-1 Erlenmeyer flasks filled with 900 ml SWM-3 and kept at 15 °C or 20 °C in 11 h light: 13 h dark and 25 °C or 30 °C in 13 h light: 11 h dark with an Illumination of 2000-2090 lux by cool white 40-W fluorescent lamps. Whole and half-fronds of tetrasporophytes used äs inocula for the second experiment were kept at an ambient temperature of 25 °C 13 :TTh. A weekly change of medium was made. After a 30-day culture period, the specific growth rate ( ) of the thallus segments expressed äs increase in length per day was determined. Maximum growth was attained at 25 °C for apical segments and 30 °C for midand basal segments. Statistical analyzes (ANOVA) show that growth is significantly different in all temperatures äs well äs between the segments. Temperature affects the growth of the segments significantly. After 21 days, the specific growth rate ( ) of the whole and half-fronds expressed äs increase in fresh weight per day was determined. Statistical analyzes (Student's t-test) indicate a highly significant difference in growth rate between free-floating half-fronds and attached half-fronds and between free-floating half-fronds and attached and free-floating whole fronds. Apical segments and half-fronds proved to be potential inocula for field cultivation and aquaculture. Seeding of inocula must commence at an appropriate temperature to enhance rapid growth, thus yielding higher production.