Clinical utility of antimicrobial susceptibility measurement plate covering formulated concentrations of various ophthalmic antimicrobial drugs.
Clinical utility of antimicrobial susceptibility measurement plate covering formulated concentrations of various ophthalmic antimicrobial drugs.
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DOI：
10.2147/opth.s108532
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Tawara A
中科院分区：
文献类型：
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作者：
Tou N;Nejima R;Ikeda Y;Hori Y;Araki-Sasaki K;Miyata K;Inoue Y;Tawara A
本研究的目的是评价眼用抗菌药物敏感性测定板SG17的临床应用价值。这是一项多中心、回顾性、观察性研究。使用眼感染患者的临床分离物，按照临床实验室和标准研究所的标准，在五个设施例行进行药敏试验。分离菌株的最低抑菌浓度由大阪大学微生物疾病研究基金会的SG17测定。对所有病例的抗菌药物使用记录和治疗过程进行评估。比较了SG17的敏感性结果和每个设施使用的常规方法。92例患者共分离到112株细菌。其中，角膜炎最常见（52.2%），其次是结膜炎（21.7%）和其他（26.1%）。所有患者的主要体征和症状均消失，表明治疗效果已达到。SG17的药敏率为98.9%，而常规方法的药敏率为30.4%。通过将临床实验室和标准协会的标准与SG17的结果相适应，我们发现91.3%的患者易感，7.6%的患者耐药。在5例患者中，最初给予耐药评估的药物没有效果，然后患者切换到最终解决的敏感评估的药物，表明临床结果与SG17一致。SG17可用于确定目前在眼科实践中使用的抗菌药物的药物敏感性。SG17可用于选择抗菌药物。
The purpose of this study was to evaluate the clinical utility of SG17, an ophthalmic antimicrobial susceptibility measurement plate. This was a multicenter, retrospective, observational study. Using clinical isolates from patients with ocular infections, drug susceptibility testing using the Clinical Laboratory and Standards Institute standards was routinely conducted at five facilities. The minimum inhibitory concentrations of the isolated strains were determined using SG17 at the Research Foundation for Microbial Diseases of Osaka University. The records of antimicrobial drugs used and the therapeutic course were evaluated for all cases. The susceptibility results from SG17 and routine methods used at each facility were compared. A total of 112 bacterial strains were isolated from 92 patients. Of these cases, keratitis was the most common (52.2%), followed by conjunctivitis (21.7%) and others (26.1%). Principal signs and symptoms resolved in all patients, indicating that therapeutic effects had been achieved. With SG17, drug susceptibility was determined in 98.9% of isolates compared with 30.4% of isolates determined using conventional methods. By adapting the Clinical Laboratory and Standards Institute standards to SG17 results, we found that 91.3% of patients were susceptible and 7.6% were resistant. In five patients, drugs with a resistant evaluation were initially administered with no effect, and the patients were then switched to drugs with a susceptible evaluation with final resolution, indicating agreement of clinical results with SG17. SG17 can be used to determine drug susceptibility to antimicrobial agents currently used in ophthalmic practice. SG17 is useful for selecting antimicrobial drugs.