Investigation of PAH biomarkers in the urine of workers exposed to hot asphalt.
Investigation of PAH biomarkers in the urine of workers exposed to hot asphalt.
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DOI：
10.1093/annhyg/mep041
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发表时间：
2009-08
期刊：
影响因子：
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通讯作者：
J. Sobus;M. McClean;R. Herrick;S. Waidyanatha;F. Onyemauwa;L. Kupper;S. Rappaport
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文献类型：
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作者：
J. Sobus;M. McClean;R. Herrick;S. Waidyanatha;F. Onyemauwa;L. Kupper;S. Rappaport
热沥青排放的空气中含有多环芳烃 (PAH) 混合物，其中包括多种致癌物质。我们调查了 20 名接触热沥青的铺路工人和 6 名未使用热沥青的道路铣削工人（参考组）的尿液中三种多环芳烃的生物标志物，即萘 (Nap)、菲 (Phe) 和芘 (Pyr)。我们的分析包括基线尿液样本以及连续三天收集的轮班后、睡前和早晨样本。我们测量了每个尿液样本中未代谢的 Nap (U-Nap) 和 Phe (U-Phe) 以及 Nap (OH-Nap)、Phe (OH-Phe) 和 Pyr (OH-Pyr) 的单羟基化代谢物。在基线样本中，摊铺机和磨坊工之间的生物标志物水平没有观察到显着差异，表明背景暴露相似。在轮班后、睡前和晨尿样本中，所有生物标志物水平之间观察到的高度成对相关性表明存在共同的暴露源。在摊铺机中，班后样品中所有生物标志物的水平均显着升高，表明接触热沥青后多环芳烃的快速吸收和消除（磨坊工人中的生物标志物水平没有升高）。摊铺机中 U-Nap、U-Phe、OH-Phe 和 OH-Pyr 水平的线性混合效应模型的结果显示，压路机操作员的生物标志物水平低于其他工人的工作分配具有显着影响。然而，没有观察到 OH-Nap 水平与工作相关的影响，这显然是由于吸烟的影响。根据摊铺机的时间回归系数估计，生物半衰期为 U-Phe 为 8 小时，U-Nap 为 10 小时，OH-Phe 和 OH-Pyr 为 13 小时，OH-Nap 为 26 小时。这些结果支持使用 U-Nap、U-Phe、OH-Phe 和 OH-Pyr（但可能不支持 OH-Nap）作为暴露于热沥青中 PAH 的短期生物标志物。
Airborne emissions from hot asphalt contain mixtures of polycyclic aromatic hydrocarbons (PAHs), including several carcinogens. We investigated urinary biomarkers of three PAHs, namely naphthalene (Nap), phenanthrene (Phe), and pyrene (Pyr) in 20 road-paving workers exposed to hot asphalt and in 6 road milling workers who were not using hot asphalt (reference group). Our analysis included baseline urine samples as well as postshift, bedtime, and morning samples collected over three consecutive days. We measured unmetabolized Nap (U-Nap) and Phe (U-Phe) as well as the monohydroxylated metabolites of Nap (OH-Nap), Phe (OH-Phe), and Pyr (OH-Pyr) in each urine sample. In baseline samples, no significant differences in biomarker levels were observed between pavers and millers, suggesting similar background exposures. In postshift, bedtime, and morning urine samples, the high pairwise correlations observed between levels of all biomarkers suggest common exposure sources. Among pavers, levels of all biomarkers were significantly elevated in postshift samples, indicating rapid uptake and elimination of PAHs following exposure to hot asphalt (biomarker levels were not elevated among millers). Results from linear mixed-effects models of levels of U-Nap, U-Phe, OH-Phe, and OH-Pyr across pavers showed significant effects of work assignments with roller operators having lower biomarker levels than the other workers. However, no work-related effect was observed for levels of OH-Nap, apparently due to the influence of cigarette smoking. Biological half-lives, estimated from regression coefficients for time among pavers, were 8 h for U-Phe, 10 h for U-Nap, 13 h for OH-Phe and OH-Pyr, and 26 h for OH-Nap. These results support the use of U-Nap, U-Phe, OH-Phe, and OH-Pyr, but probably not OH-Nap, as short-term biomarkers of exposure to PAHs emanating from hot asphalt.