The profile analysis of circular RNAs in cervical cancer.
The profile analysis of circular RNAs in cervical cancer.
复制标题
宫颈癌环状RNA谱分析
DOI：
10.1097/md.0000000000027404
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发表时间：
2021-10-01
期刊：
影响因子：
1.6
通讯作者：
Huang Q
中科院分区：
文献类型：
--
作者：
Huang J;Chen J;Huang Q
摘要宫颈癌（CC）是女性第三大常见癌症，晚期死亡率高。CC的发生和发展的潜在机制仍然是难以捉摸的。环状RNA（CircRNA）在多种生理和病理过程中发挥着重要作用。本研究的目的是鉴定与宫颈鳞状细胞癌（CSCC）显著相关的circRNA，以发现新的诊断标志物并阐明其机制基础。CSCC和配对癌旁宫颈组织的circRNA表达谱从Gene Expression Omnibus下载。利用生物信息学分析筛选差异表达的circRNA（DECRs）。通过实时定量PCR验证了hsa_circ_0000745、hsa_circ_0084927、hsa_circ_0002762、hsa_circ_0075341、hsa_circ_0007905、hsa_circ_0031027、hsa_circ_0065898、hsa_circ_0070190和hsa_circ_0078383在CC和正常宫颈组织中的表达水平。在CSCC和正常组织之间共鉴定出197个DECRs，包括87个上调和110个下调的circRNA。此外，预测上调的circRNA有37个miRNA，下调的circRNA有39个。功能分析表明，DECRs与底物粘附依赖性细胞扩散、代谢、GT3活性、蛋白调节和细胞间粘附的正调控相关。在CC的进展中起重要作用的MAPK信号通路也被富集。与计算机模拟分析一致，在CC组织中，hsa_circ_0000745、hsa_circ_0084927、hsa_circ_0002762、hsa_circ_0007905上调，hsa_circ_0078383下调（P <.001），而hsa_circ_0075341（P <.001）和hsa_circ_0031027（P =.001）显示相反的趋势。 我们确定了新的诊断和治疗CSCC的生物标志物沿着的机制基础。
Abstract Cervical cancer (CC) is the third most common cancer among women and has a high mortality rate at the advanced stage. The mechanisms underlying the development and progression of CC are still elusive. Circular RNAs (circRNAs) play an important role in various physiological and pathological processes. The aim of this study was to identify the circRNAs significantly associated with cervical squamous cell carcinoma (CSCC), in order to discover novel diagnostic markers and elucidate their mechanistic basis. The circRNA expression profiles of CSCC and paired para-cancerous cervical tissues was downloaded from the Gene Expression Omnibus. Bioinformatics analysis were used to screen for the differentially expressed circRNAs (DECRs). The expression levels of hsa_circ_0000745, hsa_circ_0084927, hsa_circ_0002762, hsa_circ_0075341, hsa_circ_0007905, hsa_circ_0031027, hsa_circ_0065898, hsa_circ_0070190, and hsa_circ_0078383 were verified in CC and normal cervical tissues by quantitative real-time PCR. A total of 197 DECRs were identified between the CSCC and normal tissues, including 87 upregulated and 110 downregulated circRNAs. In addition, 37 miRNAs were predicted for the upregulated circRNAs and 39 for the downregulated circRNAs. Functional analysis showed that the DECRs were associated with positive regulation of substrate adhesion-dependent cell spreading, metabolism, positive regulation of GTPase activity, protein regulation, and intercellular adhesion. The MAPK signaling pathway that plays a significant role in the progression of CC, was also enriched. Consistent with the in-silico analysis, hsa_circ_0000745, hsa_circ_0084927, hsa_circ_0002762, hsa_circ_0007905 were upregulated and hsa_circ_0078383 was downregulated in CC tissues (P < .001), whereas hsa_circ_0075341 (P < .001) and hsa_circ_0031027 (P = .001) showed opposite trends. We identified novel diagnostic and therapeutic biomarkers of CSCC along with the mechanistic basis.