Distribution of vasoactive intestinal polypeptide, neuropeptide-Y and substance P and their effects on insulin secretion from the in vitro pancreas of normal and diabetic rats
Distribution of vasoactive intestinal polypeptide, neuropeptide-Y and substance P and their effects on insulin secretion from the in vitro pancreas of normal and diabetic rats
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DOI：
10.1016/s0196-9781(00)00361-2
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发表时间：
2001-01-01
期刊：
影响因子：
3
通讯作者：
Singh, J
中科院分区：
文献类型：
--
作者：
Adeghate, E;Ponery, AS;Singh, J
本研究检测了糖尿病大鼠胰腺中血管活性肠肽（VIP）、神经肽-Y（NPY）和P物质（SP）的分布模式，以确定糖尿病发病后这些神经肽的数量和分布模式是否发生变化。此外，还检测了VIP、NPY和SP对正常和糖尿病大鼠胰腺胰岛素分泌的影响。糖尿病（DM）是通过腹膜内（i.p.）单剂量链脲佐菌素（STZ）160毫克/公斤体重（-1））诱导的。 4周：诱导DM后，用水合氯醛麻醉糖尿病大鼠（n = 6）和正常大鼠（n，？ = 6），取出胰腺并进行免疫组织化学和胰岛素分泌处理。糖尿病发病后，胰岛中胰岛素阳性细胞数量减少，而VIP和NPY细胞数量显着增加。正常大鼠和糖尿病大鼠的胰腺神经中 VIP、NPY 和 SP 的分布模式相似。 VIP 引起正常和糖尿病大鼠胰腺的胰岛素分泌大幅增加（P < 0.02）。 NPY 还诱导正常大鼠胰腺组织碎片的胰岛素释放显着增加（P < 0.005）。用 NPY 刺激糖尿病大鼠的胰腺组织碎片导致胰岛素释放略有但不显着增加。 SP 诱导正常大鼠胰腺的胰岛素分泌大幅增加（P < 0.005），但显着抑制糖尿病大鼠离体胰腺的胰岛素分泌（P ( 0.03））。综上所述，VIP 和 NPY 在糖尿病发病后可刺激胰腺分泌胰岛素。SP 对糖尿病大鼠胰岛素分泌的刺激作用逆转为抑制作用，(C) 2001 Elsevier Science Inc. 版权所有保留。
This study examined the pattern of distribution of vasoactive intestinal polypeptide (VIP), neuropeptide-Y (NPY) and substance P (SP) in the pancreas of diabetic rat to determine whether there are changes in the number and pattern of distribution of these neuropeptides after the onset of diabetes. Moreover, the effect of VIP, NPY and SP on insulin secretion fi om the pancreas of normal and diabetic rats was also examined. Diabetes mellitus (DM) was induced by a single dose of streptozotocin (STZ) given intraperitoneally (i.p.) 160 mg kg body weight(-1)). Four weeks: after the induction of DM, diabetic (n = 6) and normal (n,? = 6) rats were anesthetized with chloral hydrate and their pancreases removed and processed for immunohistochemistry and insulin secretion. The number of insulin-positive cells in the islets of Langerhans was reduced while that of VIP and NPY increased significantly after the onset of diabetes. The pattern of distribution of VIP, NPY and SP in the nerves innervating the pancreas was similar in both normal and diabetic rats. VIP-evoked large and significant (P < 0.02) increases in insulin secretion from the pancreas of normal and diabetic rats. NPY also induced a marked (P < 0.005) increase in insulin release from pancreatic tissue fragments of normal rat. Stimulation of pancreatic tissue fragments of diabetic rat with NPY resulted in a slight but not significant increase in insulin release. SP induced a large and significant (P < 0.005) increase in insulin secretion from the pancreas of normal rat but inhibited insulin secretion significantly (P ( 0.03) from isolated pancreas of diabetic rat. In summary, VIP and NPY can stimulate insulin secretion from the pancreas after the onset of diabetes. The stimulatory effect of SP on insulin secretion is reversed to inhibitory in diabetic rats, (C) 2001 Elsevier Science Inc. All rights reserved.