Cell Flow Reorients the Axis of Planar Polarity in the Wing Epithelium of Drosophila
Cell Flow Reorients the Axis of Planar Polarity in the Wing Epithelium of Drosophila
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DOI：
10.1016/j.cell.2010.07.042
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发表时间：
2010-09-03
期刊：
影响因子：
64.5
通讯作者：
Eaton, Suzanne
中科院分区：
文献类型：
--
作者：
Aigouy, Benoit;Farhadifar, Reza;Eaton, Suzanne
平面细胞极性(PCP)蛋白形成极化的皮质结构域，控制脊椎动物和无脊椎动物上皮中毛发和纤毛等外部结构的极性。平面极性的全球定向机制还不清楚，这里用实验和理论相结合的方法在果蝇的翅膀上进行了研究。平面极性在生长过程中出现，PCP结构域最初定位于控制生长和图案化的特征良好的组织者区域。在蛹阶段，翼铰收缩，使翼叶上皮细胞在近端-远端轴上受到各向异性的张力。这导致了定向细胞伸长、细胞重排和细胞分裂的精确模式，从而延长了叶片的近端-远端，并将平面极性与近端-远端轴重新对齐。非典型粘附素Dachsous的突变通过改变上皮细胞动力学来扰乱全球的极性模式。这一机制利用塑造组织的细胞运动来将平面极性与组织形状对准。
Planar cell polarity (PCP) proteins form polarized cortical domains that govern polarity of external structures such as hairs and cilia in both vertebrate and invertebrate epithelia. The mechanisms that globally orient planar polarity are not understood, and are investigated here in the Drosophila wing using a combination of experiment and theory. Planar polarity arises during growth and PCP domains are initially oriented toward the well-characterized organizer regions that control growth and patterning. At pupal stages, the wing hinge contracts, subjecting wing-blade epithelial cells to anisotropic tension in the proximal-distal axis. This results in precise patterns of oriented cell elongation, cell rearrangement and cell division that elongate the blade proximo-distally and realign planar polarity with the proximal-distal axis. Mutation of the atypical Cadherin Dachsous perturbs the global polarity pattern by altering epithelial dynamics. This mechanism utilizes the cellular movements that sculpt tissues to align planar polarity with tissue shape.