The genetic dependence of recombination in recD mutants of Escherichia coli.
The genetic dependence of recombination in recD mutants of Escherichia coli.
复制标题
大肠杆菌recD突变体重组的遗传依赖性。
DOI：
10.1093/genetics/120.1.37
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发表时间：
1988
期刊：
影响因子：
3.3
通讯作者：
Kolodner,RD
中科院分区：
文献类型：
--
作者：
Lovett,ST;Luisi-DeLuca,C;Kolodner,RD
RecBCD酶具有多种活性，包括解旋酶、核酸外切酶和核酸内切酶活性。编码酶的两个亚基的基因recB或recC的突变减少了许多类型重组事件的频率。编码第三个亚基的recD的突变不会减少重组，即使大多数RecBCD酶的活性严重降低。本研究探讨了recD突变体中不同类型重组的遗传依赖性。我们检测了RecBCD通路基因（recA和recC）以及RecF通路特异性基因（RecF、recJ、recN、recO、recQ、ruv和lexA）突变对偶联、转导和质粒重组以及紫外线存活的影响。在所有检测到的重组事件中，特别是涉及质粒的重组事件中，recD突变体都是高度重组的，并且recD菌株的所有重组事件都需要recA和recC。此外，与recD+菌株不同，recD菌株的染色体重组事件和紫外线对DNA损伤的修复依赖于一个RecF通路基因recJ。只有一部分重组事件受到ruv、recN、recQ、recO和lexA突变的影响。
RecBCD enzyme has multiple activities including helicase, exonuclease and endonuclease activities. Mutations in the genes recB or recC, encoding two subunits of the enzyme, reduce the frequency of many types of recombinational events. Mutations in recD, encoding the third subunit, do not reduce recombination even though most of the activities of the RecBCD enzyme are severely reduced. In this study, the genetic dependence of different types of recombination in recD mutants has been investigated. The effects of mutations in genes in the RecBCD pathway (recA and recC) as well as the genes specific for the RecF pathway (recF, recJ, recN, recO, recQ, ruv and lexA) were tested on conjugational, transductional and plasmid recombination, and on UV survival. recD mutants were hyper-recombinogenic for all the monitored recombination events, especially those involving plasmids, and all recombination events in recD strains required recA and recC. In addition, unlike recD+ strains, chromosomal recombination events and the repair of UV damage to DNA in recD strains were dependent on one RecF pathway gene, recJ. Only a subset of the tested recombination events were affected by ruv, recN, recQ, recO and lexA mutations.