Single-Particle Tracking of Hepatitis B Virus-like Vesicle Entry into Cells
Single-Particle Tracking of Hepatitis B Virus-like Vesicle Entry into Cells
复制标题
乙型肝炎病毒样囊泡进入细胞的单粒子追踪
DOI：
10.1002/smll.201002020
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发表时间：
2011-05-09
期刊：
影响因子：
13.3
通讯作者：
Wang, Hongda
中科院分区：
文献类型：
--
作者：
Hao, Xian;Shang, Xin;Wang, Hongda
以乙肝病毒表面抗原--乙型肝炎表面抗原为模型，在单粒子水平上进行成像和示踪，研究单膜病毒感染活细胞的机制和动力学。通过监测HBs Ag颗粒的荧光指示剂，发现HBs Ag通过小窝蛋白介导的内吞途径进入细胞。对活细胞中单个乙肝表面抗原颗粒的跟踪显示，内化的乙肝表面抗原颗粒的运动异常依赖于肌动蛋白，但不依赖于微管。定量分析了活细胞内乙肝表面抗原颗粒的运动性。这些结果可能会解决长期存在的争论，即乙肝病毒是直接打破质膜屏障，还是依靠内吞作用将其基因组运送到细胞内，以及病毒如何在细胞内运动。
HBsAg, the surface antigen of the hepatitis B virus (HBV), is used as a model to study the mechanisms and dynamics of a single-enveloped virus infecting living cells by imaging and tracking at the single-particle level. By monitoring the fluorescent indicator of HBsAg particles, it is found that HBsAg enters cells via a caveolin-mediated endocytic pathway. Tracking of individual HBsAg particles in living cells reveals the anomalously actin-dependent but not microtubule-dependent motility of the internalized HBsAg particle. The motility of HBsAg particles in living cells is also analyzed quantitatively. These results may settle the long-lasting debate of whether HBV directly breaks the plasma membrane barrier or relies on endocytosis to deliver its genome into the cell, and how the virus moves in the cell.