Genome-Wide Identification and Characterization of the AREB/ABF/ABI5 Subfamily Members from Solanum tuberosum
Genome-Wide Identification and Characterization of the AREB/ABF/ABI5 Subfamily Members from Solanum tuberosum
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马铃薯 AREB/ABF/ABI5 亚家族成员的全基因组鉴定和表征
DOI：
10.3390/ijms20020311
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发表时间：
2019-01-02
影响因子：
5.6
通讯作者：
Song, Botao
中科院分区：
文献类型：
--
作者：
Liu, Tengfei;Zhou, Tingting;Song, Botao
脱落酸（ABA）在植物发育和适应环境胁迫中起着至关重要的作用。ABA响应元件结合蛋白/ abre结合因子和ABA不敏感5 （AREB/ABF/ABI5）基因亚家族成员属于碱性结构域/亮氨酸拉链（bZIP）转录因子家族，它们通过调控靶基因的表达参与ABA介导的信号通路。然而，关于马铃薯（Solanum tuberosum） AREB/ABF/ABI5亚家族成员的信息仍然很少。本研究在马铃薯基因组中鉴定了7个推测的AREB/ABF/ABI5成员。序列比对显示，这些成员具有较高的蛋白质序列相似性，特别是在bZIP区域，表明它们可能在调节基因表达方面具有重叠作用。亚细胞定位分析表明，7个AREB/ABF/ABI5成员均定位于细胞核。酵母的转激活活性分析表明，这些AREB/ABF/ABI5成员具有不同的转录活性。电泳迁移迁移试验（EMSA）证实，所有这些AREB/ABF/ABI5成员在体外都可能与ABRE具有亲和力。这些AREB/ABF/ABI5基因的表达模式表明它们对ABA或渗透胁迫有不同程度的响应。大部分AREB/ABF/ABI5基因在匍匐茎肿胀过程中被诱导。总的来说，这些结果首次提供了马铃薯AREB/ABF/ABI5亚家族的全面鉴定，并将有助于在未来的工作中进一步表征这些亚家族成员的功能。
Abscisic acid (ABA) plays crucial roles in plant development and adaption to environmental stresses. The ABA-responsive element binding protein/ABRE-binding factor and ABA INSENSITIVE 5 (AREB/ABF/ABI5) gene subfamily members, which belong to the basic domain/leucine zipper (bZIP) transcription factors family, participate in the ABA-mediated signaling pathway by regulating the expression of their target genes. However, information about potato (Solanum tuberosum) AREB/ABF/ABI5 subfamily members remains scarce. Here, seven putative AREB/ABF/ABI5 members were identified in the potato genome. Sequences alignment revealed that these members shared high protein sequence similarity, especially in the bZIP region, indicating that they might possess overlapping roles in regulating gene expression. Subcellular localization analysis illustrated that all seven AREB/ABF/ABI5 members were localized in the nucleus. Transactivation activity assays in yeast demonstrated that these AREB/ABF/ABI5 members possessed distinct transcriptional activity. Electrophoretic mobility shift assays (EMSA) confirmed that all of these AREB/ABF/ABI5 members could have an affinity to ABRE in vitro. The expression patterns of these AREB/ABF/ABI5 genes showed that they were in response to ABA or osmotic stresses in varying degrees. Moreover, most AREB/ABF/ABI5 genes were induced during stolon swelling. Overall, these results provide the first comprehensive identification of the potato AREB/ABF/ABI5 subfamily and would facilitate further functional characterization of these subfamily members in future work.