Regulation of lignin biosynthesis in fruit pericarp hardening of mangosteen (Garcinia mangostana L.) after impact
Regulation of lignin biosynthesis in fruit pericarp hardening of mangosteen (Garcinia mangostana L.) after impact
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DOI：
10.1016/j.postharvbio.2014.06.004
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发表时间：
2014-11-01
影响因子：
7
通讯作者：
Ketsa, Saichol
中科院分区：
文献类型：
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作者：
Kamdee, Chanattika;Imsabai, Wachiraya;Ketsa, Saichol
新鲜山竹（Garcinia mangostana L.）果实的果皮硬化是对收获期间和收获后处理不当的快速反应。撞击后受损山竹果皮的硬度、木质素含量和木质素成分（G 和 S 木质素）迅速增加，而总游离酚含量下降。与保存在环境空气中的水果相比，在撞击后对水果施用氮气可以减少这些影响。大部分编码山竹木质素生物合成途径的基因和全长 MYB 转录因子 (R2R3 MYB) 均已分离。使用 qPCR 的表达分析表明，在编码木质素生物合成酶的基因中，只有 GmCCoAMT 和 GmF5H 在撞击后增加，并且与硬度和木质素含量的增加相关。与应激相关的 R2R3 MYB 转录因子的转录水平因冲击而显着增加，并因氮含量升高而延迟。这些结果表明，山竹在撞击后果皮硬化是由于木质素生物合成途径后期步骤的快速转录激活，可能是通过 R2R3 MYB 等转录因子的上调所致。 (C) 2014 Elsevier B.V. 保留所有权利。
Pericarp hardening in fresh mangosteen (Garcinia mangostana L.) fruit is a rapid response to mishandling during and after harvest. Firmness, lignin content and lignin composition (G and S lignin) increased rapidly, while total free phenolic content decreased in damaged mangosteen pericarp following impact. Application of nitrogen to the fruit after impact reduced these effects, compared with fruit kept in ambient air. The majority of the genes encoding the mangosteen lignin biosynthetic pathway, and a full length MYB transcription factor (R2R3 MYB), were isolated. Expression analysis using qPCR showed that of the genes encoding enzymes in lignin biosynthesis, only GmCCoAMT and GmF5H increased after impact and correlated with increases in firmness and lignin content. The transcript level of a stress-related R2R3 MYB transcription factor was significantly increased by impact, and delayed by elevated nitrogen. These results suggest that pericarp hardening of mangosteen after impact is due to rapid transcriptional activation of late steps of the lignin biosynthetic pathway, potentially via up-regulation of transcription factors such as R2R3 MYBs. (C) 2014 Elsevier B.V. All rights reserved.