TRANSCRIPTIONAL ENHANCERS FROM SV40 AND POLYOMA-VIRUS SHOW A CELL TYPE PREFERENCE
TRANSCRIPTIONAL ENHANCERS FROM SV40 AND POLYOMA-VIRUS SHOW A CELL TYPE PREFERENCE
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DOI：
10.1093/nar/10.24.7965
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发表时间：
1982-01-01
影响因子：
14.9
通讯作者：
SCHAFFNER, W
中科院分区：
文献类型：
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作者：
DEVILLIERS, J;OLSON, L;SCHAFFNER, W
在猴病毒SV 40和小鼠多瘤病毒中均发现了转录“增强子”。在这里，我们报告说，这些增强子表现出细胞类型的偏好。这是通过（i）测定含有自身增强子或SV 40病毒增强子的多瘤DNA的T抗原表达和病毒DNA复制，以及（ii）将两个增强子元件中的任一个连接到兔β1-珠蛋白基因并测量小鼠和灵长类动物细胞中的瞬时珠蛋白表达来完成的。所有试验的结果都是一致的：在小鼠细胞中，多瘤增强子比SV 40增强子稍微更有效。然而，在灵长类动物细胞中，SV 40增强子诱导的基因表达水平比多瘤增强子高4至6倍。
Transcriptional “enhancers” have been identified in both the monkey virus SV40 and in the mouse polyoma virus. Here we report that these enhancers show a cell type preference. This was done, (i) by assaying for T antigen expression and viral DNA replication of polyoma DNA which contains its own enhancer or the enhancer of SV40 virus, and (ii) by linking either of the two enhancer elements to the rabbit β1-globin gene and measuring transient globin expression in mouse and primate cells. The results were consistent in all the assays: In mouse cells the polyoma enhancer is slightly more effective than the SV40 enhancer. However, in primate cells the SV40 enhancer induces a four to six fold higher level of gene expression than does the polyoma enhancer.