Cross-Species Surveillance of Leptospira in Domestic and Pen-Domestic Animals in Mahalla City, Gharbeya Governorate, Egypt
Cross-Species Surveillance of Leptospira in Domestic and Pen-Domestic Animals in Mahalla City, Gharbeya Governorate, Egypt
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DOI：
10.4269/ajtmh.2011.10-0393
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发表时间：
2011-03-01
影响因子：
3.3
通讯作者：
Pimentel, Guillermo
中科院分区：
文献类型：
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作者：
Felt, Stephen A.;Wasfy, Momtaz O.;Pimentel, Guillermo
在Mahalla市（下埃及）对179只动物（黑鼠、狗、绵羊、水牛、牛、驴、黄鼠狼和猫）进行了钩端螺旋体感染调查。采集血液、尿液和肾脏，并通过培养、显微镜凝集试验（MAT）和/或聚合酶链反应（PCR）进行检测。在大鼠中，26%通过PCR检测为阳性，包括7%通过L.问号线虫血清型流感伤寒型、产热菌属和黄疸出血型。L.在埃及的三只大鼠中首次分离到波洛尼卡波氏疏螺旋体血清型。在11%的大鼠和12%的犬中观察到MAT滴度>= 1：800。L.用上述方法检测羊和驴的钩端螺旋体病均为阴性。水牛和牛的血清阳性率分别为20%和44%。数据表明，几种致病血清型正在动物中传播，这可能会造成接触风险，并导致急性发热性疾病的高发率。
A survey of 179 animals (black rats, dogs, sheep, buffaloes, cattle, donkeys, weasels, and cats) for Leptospira infection was conducted in Mahalla City (Lower Egypt). Blood, urine, and kidney were collected and tested by culture, microscopic agglutination test (MAT), and/or polymerase chain reaction (PCR). Among rats, 26% were positive by PCR, including 7% that were also positive by culture for L. interrogans serovars Grippotyphosa, Pyrogenes, and Icterohaemorrhagiae. L. borpetersenii serovar Polonica was isolated for the first time in Egypt in three rats. MAT titers >= 1:800 were observed in 11% of rats and 12% of dogs. L. interrogans serovar Grippotyphosa was detected in one cat. Sheep and donkeys were negative for leptospirosis by all methods. Buffaloes and cattle were seropositive in 20% and 44% of animals, respectively. Data indicate that several pathogenic serovars are circulating in the animals, which may pose exposure risks and account for high rates of acute febrile illness.