Toll-like receptor 2 is expressed on the intestinal M cells in swine
Toll-like receptor 2 is expressed on the intestinal M cells in swine
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DOI：
10.1016/j.bbrc.2005.03.017
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发表时间：
2005-05-06
影响因子：
3.1
通讯作者：
Saito, T
中科院分区：
文献类型：
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作者：
Tohno, M;Shimosato, T;Saito, T
Toll样受体(TLR)2与多种微生物细胞壁成分结合。本研究采用实时定量聚合酶链式反应、Western blotting、免疫组织化学和流式细胞术等方法，研究了TLR2在成年猪肠道相关淋巴组织中的表达模式。TLR2基因在成年猪肠系膜淋巴结(MLN)和Peyer‘s Patches(PPS)中优先表达。在这两种组织中的表达分别是脾组织的15倍和9倍。Western blotting进一步证实TLR2蛋白在MLN和PPS中高表达。有趣的是，表达TLR2的细胞不仅存在于T细胞和B细胞等免疫细胞中，而且也存在于膜(M)细胞中。此外，TLR2和细胞角蛋白18的双重免疫染色显示，TLR2不仅在囊状M细胞的胞浆中有强表达，而且在顶膜上也有强表达。这些结果表明，MLN和PPS上的TLR2使寄主能够对各种细胞壁成分做出反应。此外，TLR2作为一种模式识别受体的潜在功能及其在细胞中的分布表明，TLR2在M细胞的配体特异性转运和转运中发挥着重要作用。(C)2005 Elsevier Inc.保留所有权利。
The Toll-like receptor (TLR) 2 binds a wide variety of microbial cell wall components. In this study, we investigated the expression pattern of TLR2 in adult swine gut-associated lymphoid tissues using real-time quantitative PCR, Western blotting, immunohistochemistry, and flow cytometric analysis. The mRNA for TLR2 was preferentially expressed in the mesenteric lymph nodes (MLNs) and Peyer's patches (Pps) of adult swine. Expression in these two tissues was approximately 15- and 9-fold higher than that of spleen, respectively. Western blotting further confirmed that the TLR2 protein was highly expressed in the MLNs and Pps. Interestingly, TLR2-expressing cells were found not only in immune cells, such as T cells and B cells, but also in membranous (M) cells. In addition, double immunostaining for TLR2 and cytokeratin 18 revealed that TLR2 was strongly expressed not only in the cytoplasm but also in the apical membrane of the pocket-like M cells. These results indicate that TLR2 on the MLNs and Pps enable the host defense to respond to a variety of cell wall components. Furthermore, the potential function of TLR2 as a pattern recognition receptor and its cellular distribution suggest that TLR2 plays an important role in ligand-specific transcytosis and transport in M cells. (c) 2005 Elsevier Inc. All rights reserved.