Behavior of stem cell spermatogonia in the adult rat irradiated testis.
Behavior of stem cell spermatogonia in the adult rat irradiated testis.
复制标题
成年大鼠受辐射睾丸中干细胞精原细胞的行为。
DOI：
10.1095/biolreprod19.4.747
复制
发表时间：
1978
影响因子：
3.6
通讯作者：
C. Huckins
中科院分区：
文献类型：
--
作者：
C. Huckins
在几项成年大鼠受辐射睾丸的研究中，已鉴定、定量和表征了抗辐射精原细胞。在第一项研究中，成年雄性大鼠，暴露于330 R的全身钴辐射，在几个时间间隔杀死长达51天。这个剂量选择性地破坏了几乎所有的精原细胞，除了一些被确定为A型干细胞的辐射抗性元素。这些精原细胞的最小数量发生在照射后10至13天，值为对照组的12-15%。在同一时期，精母细胞和细线期前精母细胞的分化率仅为对照组的1%左右.从13天开始，作为类型的幸存者和他们的后代在增殖室进行增强有丝分裂活动，导致最终恢复的精原细胞群体的全尺寸照射后51天。在第二组实验中，成年雄性大鼠注射（cid：1）3 HI TdR，随后用300 R钴全身照射或保留作为未照射对照。在照射后6至15天，分析对照组和照射组睾丸的生精小管。在每个时间间隔，标记的抗辐射A5型精原细胞中发现的上皮细胞。这些细胞在各阶段优先掺入标记物。结论是辐照处于活跃的细胞周期中，并启动了耗竭上皮的修复。这些抗辐射的A5型细胞的标记模式与正常睾丸中长周期的A5型精原细胞的标记模式相同。虽然它们的精确行为仍不完全，但这些长周期A5精原细胞在确保正常和辐照大鼠生殖细胞系的永久存在方面发挥着重要作用。
Radioresistant spermatogonia have been identified, quantitated and characterized in several studies of irradiated testes of the adult rat. In the first study, adult male rats, exposed to 330 R of whole body cobalt irradiation, were killed at several intervals up to 51 days. This dose selectively destroyed almost all spermatogonia except for some radioresistant elements that were identified as type As stem cells. Minimal numbers of these spermatogonia occurred between 10 and 13 days after irradiation when values were 12-15% of controls. In the same period, differentiating sperms- togonia and preleptotene spermatocytes were only about 1% of control figures. Beginning at 13 days, the type As survivors and their progeny in the proliferating compartment underwent enhanced mitotic activity which led to the eventual restoration of the spermatogonial population to its full size by 51 days after irradiation. In a second set of experiments, adult male rats were injected with (cid:1)3 HI TdR and subsequently either irradiated with 300 R cobalt whole body irradiation or retained as nonirradiated controls. Seminiferous tubules from both control and irradiated testes were analyzed between 6 and 15 days after irradiation. At each interval, labeled radioresistant type A5 spermatogonia were found in the epithelium. These had preferentially incorporated label during stages It was concluded the irradia- tion were in active cell cycle and initiated repair of the depleted epithelium. Moreover, the labeling pattern of these radioresistant type A5 cells was identical to that of the long cycling A5 spermato- gonia found in normal testes. While their precise behavior is still incompletely charted, these long cycling A5 spermatogonial cells play a fundamental role in assuring the perpetuation of the germ cell line in both normal and irradiated rats.