Requirement of a Tsp2-Type Tetraspanin for Laccase Repression and Stress Resistance in the Basidiomycete Cryptococcus neoformans
Requirement of a Tsp2-Type Tetraspanin for Laccase Repression and Stress Resistance in the Basidiomycete Cryptococcus neoformans
复制标题
担子菌新生隐球菌中漆酶抑制和应激抵抗所需的 Tsp2 型四跨膜蛋白
DOI：
10.1128/aem.06072-11
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发表时间：
2012-01-01
影响因子：
4.4
通讯作者：
Zhu, Xudong
中科院分区：
文献类型：
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作者：
Li, Zhongming;Bi, Jiannan;Zhu, Xudong
摘要真菌漆酶在工业上有着广泛的应用。在许多真菌中，漆酶的表达常常被初级碳源葡萄糖抑制。其基本依据在很大程度上尚不清楚。在这里，我们证明了一个基因，TSP 2 -1，所需的漆酶阻遏葡萄糖在担子菌隐球菌新生。TSP 2 -1编码Tsp 2型四跨膜蛋白。TSP 2 -1的破坏导致黑色素的恒定形成和漆酶基因LAC 1的表达。这种去阻遏表型可被10 mM外源性环腺苷酸（cAMP）抑制。突变体tsp 2 -1Δ中的包膜缺陷也被cAMP修复。结果表明Tsp 2 -1与cAMP依赖性蛋白激酶A（PKA）途径的相互作用，该途径已被证明可调节该真菌中的漆酶阻遏和荚膜生物合成。TSP 2 -1的其他作用，例如，在维持细胞膜完整性和抗胁迫性方面也被定义。这项工作揭示了Tsp 2 -1依赖的葡萄糖阻遏在C。新人类本文首次报道了Tsp 2型四跨膜蛋白Tsp 2 -1的功能。
ABSTRACT Fungal laccases have been widely used in industry. The expression of laccase often is repressible by the primary carbon source glucose in many fungi. The underlying basis is largely unclear. We demonstrate here that a gene, TSP2-1, was required for laccase repression by glucose in the basidiomycete Cryptococcus neoformans. TSP2-1 encodes a Tsp2-type tetraspanin. The disruption of TSP2-1 resulted in constant melanin formation and the expression of the laccase gene LAC1. This derepression phenotype was restorable by 10 mM exogenous cyclic AMP (cAMP). A capsule defect in the mutant tsp2-1Δ also was restored by cAMP. The results indicate an interaction of Tsp2-1 with the cAMP-dependent protein kinase A (PKA) pathway that has been shown to modulate laccase repression and capsule biosynthesis in this fungus. Other roles of TSP2-1, e.g., in maintaining cell membrane integrity and stress resistance, also were defined. This work reveals a Tsp2-1-dependent glucose repression in C. neoformans. The function of Tsp2-type tetraspanin Tsp2-1 is described for the first time.