Localization of the sentinel lymph node in tongue VX2 carcinoma via indirect CT lymphography combined with methylene blue dye injection
Localization of the sentinel lymph node in tongue VX2 carcinoma via indirect CT lymphography combined with methylene blue dye injection
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DOI：
10.3109/00016480903253595
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发表时间：
2010-04-01
影响因子：
1.4
通讯作者：
Zhou, Liang
中科院分区：
文献类型：
--
作者：
Wu, Haitao;Ying, Haiyue;Zhou, Liang
结论：间接CT淋巴造影（CT-LG）结合蓝色染料注射可定位舌癌前哨淋巴结（SLN）。目的：应用间接CT-LG结合美蓝注射对舌VX 2癌前哨淋巴结进行定位。方法：将18只家兔分为转移组、增生组和对照组。间接CT-LG通过将碘海醇注射到舌粘膜下层进行。在注射后1、5和15 min采集CT图像。间接CT-LG后24 h在同一区域注射亚甲蓝，然后进行SLN鉴定和组织病理学检查。结果：SLN显示率为100%。前哨淋巴结的位置确定使用CT淋巴造影证实了亚甲蓝染料。对照组和增生增强组SLN均呈圆形或椭圆形，无充盈缺损。对照组家兔在注射后1、5和15 min的SLN衰减值分别为689.2 +/- 55.4 HU、278.4 +/- 33.5 HU和71.7 +/- 9.2 HU。增生兔的数值分别为877.4 +/- 69.4 HU、352.5 +/- 43.3 HU和80.2 +/- 11.5 HU。仅在转移组的同侧转移SLN中观察到充盈缺损。在注射碘海醇后1、5和15 min，转移性SLN的CT衰减值分别为687.4 +/- 55.6 HU、535.1 +/- 86.6 HU和282.3 +/- 19.4 HU。在注射后5分钟（p < 0.001）和15分钟（p <0.001），转移淋巴结的衰减值显著大于对照或增生淋巴结的衰减值。
Conclusions: Indirect computed tomography lymphography (CT-LG) combined with blue dye injection can locate the sentinel lymph node (SLN) in tongue carcinoma. Objective: To localize the SLN in tongue VX2 carcinoma using indirect CT-LG combined with methylene blue injection. Methods: Eighteen rabbits were placed into three groups: metastasis, hyperplasia, and control. Indirect CT-LG was performed with an iohexol injection into the tongue submucosa. CT images were acquired at 1, 5, and 15 min after injection. Methylene blue was injected into the same area 24 h after indirect CT-LG, followed by SLN identification and histopathological examination. Results: SLNs were visualized with an identification rate of 100%. SLN location identified using CT lymphography was confirmed by methylene blue dye. Control and hyperplastic enhanced SLNs were round or oval without any filling defects. Control rabbits had enhanced SLN attenuation values of 689.2 +/- 55.4 HU, 278.4 +/- 33.5 HU, and 71.7 +/- 9.2 HU at 1, 5, and 15 min after injection, respectively. Hyperplastic rabbits had values of 877.4 +/- 69.4 HU, 352.5 +/- 43.3 HU, and 80.2 +/- 11.5 HU. Filling defects were only observed in the ipsilateral metastatic SLNs of the metastasis group. CT attenuation values of the metastatic SLNs were 687.4 +/- 55.6 HU, 535.1 +/- 86.6 HU, and 282.3 +/- 19.4 HU at 1, 5, and 15 min, respectively, after iohexol injection. Metastatic lymph node attenuation values were significantly greater than those of the control or hyperplastic nodes at 5 (p < 0.001) and 15 min (p < 0.001) after injection.