Serine substitutions caused by an ochre suppressor in yeast.
Serine substitutions caused by an ochre suppressor in yeast.
复制标题
由酵母中的赭石抑制剂引起的丝氨酸取代。
DOI：
10.1016/0022-2836(75)90324-1
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发表时间：
1975
影响因子：
5.6
通讯作者：
F. Sherman
中科院分区：
文献类型：
--
作者：
S. Liebman;J. Stewart;F. Sherman
酵母中的抑制子suq5与赤褐色（UAA）突变体scyc1 - 2和cyc1 - 72偶联时，可以产生正常量的约10 - 20%的isol -细胞色素。同染色质在与赭色密码子对应的位置上含有丝氨酸残基。SUQ5仅在具有非孟德尔因子ψ+的菌株中有效，尽管来自ψ−SUQ5 cyc1 - 72菌株的低量抑制的异-l-细胞色素也被证明在赭色位点含有丝氨酸。因此，sussuq5不同于酵母中其他八个特征化的UAA抑制因子，这些抑制因子先前被证明在黄褐色位点插入酪氨酸残基（Gilmoreet al., 1971），并且仅在具有非孟德尔因子ψ -的菌株中有效，因为它们通常导致在ψ+状态下无法生存。与酪氨酸插入抑制子一样，suq5也可以作用于另一种赭色等位基因c1 - 9，但效率非常低，约为0.4%，而它似乎对琥珀色（UAG）突变体完全不起作用。发现suq5距XVI染色体tyr7 6.4 cM （cM）。推测suq5的基因产物为丝氨酸tRNA。
The suppressorSUQ5in yeast can cause the production of approximately 10 to 20% of the normal amount of iso-l-cytochromecwhen coupled to the ochre (UAA) mutantscyc1–2andcyc1–72. The iso-l-cytochromesccontain residues of serine at positions that correspond to the sites of the ochre codons.SUQ5is efficient only in strains having the non-Mendelian factorψ+, although the low amount of suppressed iso-l-cytochromecfrom aψ−SUQ5 cyc1–72strain was also shown to contain serine at the ochre site. ThusSUQ5differs from the eight other characterized suppressors of UAA in yeast, which were previously shown to insert residues of tyrosine at ochre sites (Gilmoreet al., 1971) and which are only effective in strains haying the non-Mendelian factorψ−, since they generally cause inviability in theψ+state. Like the tyrosine-inserting suppressors,SUQ5can also act on another ochre allelecyc1–9, but with a very low efficiency of approximately 0.4%, while it does not appear to act at all on amber (UAG) mutants.SUQ5was found to be 6.4 cM (centiMorgans) fromtyr7on chromosome XVI. It is suggested that the gene product ofSUQ5is serine tRNA.