The downy mildew effector proteins ATR1 and ATR13 promote disease susceptibility in Arabidopsis thaliana
The downy mildew effector proteins ATR1 and ATR13 promote disease susceptibility in Arabidopsis thaliana
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DOI：
10.1105/tpc.107.054262
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发表时间：
2007-12-01
期刊：
影响因子：
11.6
通讯作者：
Jones, Jonathan D. G.
中科院分区：
文献类型：
--
作者：
Sohn, Kee Hoon;Lei, Rita;Jones, Jonathan D. G.
霜霉病（Hyaloperonospora parasitica）效应蛋白ATR 1和ATR 13触发RPP 1-Nd/WsB-和RPP 13-Nd依赖的抗性，分别在拟南芥。为了更好地了解这些效应子在H.为了在拟南芥属种质上获得寄生虫分离物，我们开发了一种新的递送系统，其使用假单胞菌III型分泌，通过ATR与假单胞菌效应蛋白AvrRPS4的N末端的融合。当从番茄疫霉致病变种（Pst）DC 3000中传递时，ATR 1和ATR 13分别在携带RPP1-Nd/WsB或RPP13-Nd的拟南芥中触发过敏反应（HR）和对细菌病原菌的抗性。此外，ATR 1和ATR 13的多个等位基因赋予增强的毒力Pst DC3000对敏感的拟南芥种质。我们的结论是，ATR 1和ATR 13积极有助于宿主细胞内的病原体毒力。两个ATR13等位基因抑制细菌PAMP（病原体相关的分子模式）触发胼胝质沉积在敏感的拟南芥时，DC3000三角洲CEL突变体交付。此外，ATR13的另一个等位基因在植物细胞中的表达抑制PAMP触发的活性氧的产生除了胼胝质沉积。有趣的是，尽管Wassilewskija（Ws-0）对H.寄生虫分离株Emco5、ATR 13（Emco5）在通过Pst DC 3000递送时触发了Ws-0上的局部免疫，包括HR。我们建议增加一个H.寄生虫Emco5效应子可能抑制ATR 13触发的免疫。
The downy mildew (Hyaloperonospora parasitica) effector proteins ATR1 and ATR13 trigger RPP1-Nd/WsB- and RPP13-Nd-dependent resistance, respectively, in Arabidopsis thaliana. To better understand the functions of these effectors during compatible and incompatible interactions of H. parasitica isolates on Arabidopsis accessions, we developed a novel delivery system using Pseudomonas syringae type III secretion via fusions of ATRs to the N terminus of the P. syringae effector protein, AvrRPS4. ATR1 and ATR13 both triggered the hypersensitive response (HR) and resistance to bacterial pathogens in Arabidopsis carrying RPP1-Nd/WsB or RPP13-Nd, respectively, when delivered from P. syringae pv tomato (Pst) DC3000. In addition, multiple alleles of ATR1 and ATR13 confer enhanced virulence to Pst DC3000 on susceptible Arabidopsis accessions. We conclude that ATR1 and ATR13 positively contribute to pathogen virulence inside host cells. Two ATR13 alleles suppressed bacterial PAMP ( for Pathogen-Associated Molecular Patterns)-triggered callose deposition in susceptible Arabidopsis when delivered by DC3000 Delta CEL mutants. Furthermore, expression of another allele of ATR13 in plant cells suppressed PAMP-triggered reactive oxygen species production in addition to callose deposition. Intriguingly, although Wassilewskija (Ws-0) is highly susceptible to H. parasitica isolate Emco5, ATR13(Emco5) when delivered by Pst DC3000 triggered localized immunity, including HR, on Ws-0. We suggest that an additional H. parasitica Emco5 effector might suppress ATR13-triggered immunity.