Measurement of piperaquine in plasma by liquid chromatography with ultraviolet absorbance detection
Measurement of piperaquine in plasma by liquid chromatography with ultraviolet absorbance detection
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DOI：
10.1016/s1570-0232(03)00209-5
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发表时间：
2003-07-05
影响因子：
3
通讯作者：
Ilett, KF
中科院分区：
文献类型：
--
作者：
Hung, TY;Davis, TME;Ilett, KF
阿喹喹（PQ）是一种抗疟药物，由于寄生虫对标准治疗的耐药性，与青蒿素衍生物联合使用的情况再次出现。其药代动力学特性尚未表征。采用沃茨XTerra（TM）RP 18色谱柱，以7%乙腈水溶液（含0.025%三氟乙酸、0.1% NaCl和0.008%三乙胺）为移动的流动相，在340 nm处进行UV检测，开发了血浆中PQ的含量测定。该测定在高达1000 μ g/l时呈线性。日内和日间相对标准偏差为
Piperaquine (PQ) is an antimalarial drug enjoying a resurgence of use in combination with an artemisinin derivative because of parasite resistance to standard treatments. Its pharmacokinetic properties have not been characterised. An assay for PQ in plasma was developed using solvent extraction and liquid chromatographic separation on a Waters XTerra(TM) RP18 column, with a mobile phase of 7% acetonitrile in water (containing 0.025% trifluoroacetic acid, 0.1% NaCl and 0.008% triethylamine) and UV detection at 340 nm. The assay was linear up to 1000 mug/l. Intra- and inter-day relative standard deviations were