Androgen receptor mRNA expression in Xenopus laevis CNS: sexual dimorphism and regulation in laryngeal motor nucleus.
Androgen receptor mRNA expression in Xenopus laevis CNS: sexual dimorphism and regulation in laryngeal motor nucleus.
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非洲爪蟾 CNS 中雄激素受体 mRNA 表达：喉运动核的性二态性和调节。
DOI：
10.1002/(sici)1097-4695(199608)30:4
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Kelley,DB
中科院分区：
文献类型：
--
作者：
Pérez,J;Cohen,MA;Kelley,DB
利用Northern分析、原位杂交和核酸酶保护试验，检测了幼年非洲爪蟾CNS中雄激素受体信使RNA（AR mRNA）的表达和调控。只有一种AR mRNA亚型在X中表达。 Northern 印迹分析显示，laevisis 在 CNS 中转录。核酸酶保护测定表明，AR mRNA 在脑干中的表达高于端脑和间脑。尽管 AR mRNA 的表达在整个 CNS 中广泛存在，但脑神经核 IX-X (N. IX-X) 和脊髓的细胞在其细胞质中显示出最高的原位杂交信号。使用辣根过氧化物酶和地高辛标记的 AR 探针进行双重标记，揭示喉部和前脊髓运动神经元表达 AR mRNA。男性 N.IX-X 中表达 AR mRNA 的细胞多于女性。两性性腺切除后，N.IX-X 中 AR 表达细胞的数量都会减少，而二氢睾酮 (DHT) 治疗 1 个月可以逆转这种效应。在核酸酶保护测定中，DHT 处理动物大脑中 AR mRNA 表达的增加也很明显。 AR 表达细胞数量的性别差异以及运动核中 AR mRNA 表达的激素调节可能会影响致力于性别分化行为的神经肌肉系统。 © 1996 约翰威利父子公司。
Using Northern analysis,in situhybridization, and nuclease protection assays, the expression and regulation of androgen receptor messenger RNA (AR mRNA) was examined in the CNS of juvenileXenopus laevis.Only one of the AR mRNA isoforms expressed inX. laevisis transcribed in the CNS as shown by Northern blot analysis. Nuclease protection assays demonstrate that the expression of AR mRNA is higher in the brain stem than in the telencephalon and diencephalon. Although expression of AR mRNA is widespread throughout the CNS, cells of cranial nerve nucleus IX‐X (N. IX‐X) and spinal cord display the highestin situhybridization signals in their cytoplasm. Double labeling using horseradish peroxidase and digoxigenin labeled AR probes reveals that laryngeal and anterior spinal cord motor neurons express AR mRNA. More cells express AR mRNA in N. IX‐X of males than of females. The number of AR expressing cells in N. IX‐X decreases following gonadectomy in both sexes, and dihydrotestosterone (DHT) treatment for 1 month reverses this effect. Increased expression of AR mRNA in the brain of DHT treated animals is also apparent in nuclease protection assays. Sex differences in number of AR expressing cells and hormone regulation of AR mRNA expression in motor nuclei may influence neuromuscular systems devoted to sexually differentiated behaviors. © 1996 John Wiley & Sons, Inc.