Evaluation of parameters influencing the molecular delivery by biodegradable microsphere-mediated perforation using femtosecond laser
Evaluation of parameters influencing the molecular delivery by biodegradable microsphere-mediated perforation using femtosecond laser
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DOI：
10.1117/1.jbo.19.1.015003
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发表时间：
2014
影响因子：
3.5
通讯作者：
Tatsuki Mitsuhashi;M. Terakawa
中科院分区：
文献类型：
--
作者：
Tatsuki Mitsuhashi;M. Terakawa
抽象。为开发高效的分子传递技术，对影响生物可降解微球介导飞秒激光穿孔传递速率的关键参数进行了详细研究。用弱聚焦飞秒激光脉冲照射与细胞膜结合的球形聚乳酸微球，使细胞膜穿孔。详细研究了异硫氰酸荧光素-葡聚糖和荧光二氧化硅颗粒向A431细胞的递送。激光脉冲照射次数的增加导致了传输速率的增加。递送速率取决于荧光二氧化硅颗粒的尺寸和功能化，其中直径为100 nm的二氧化硅颗粒能够被递送到20%的照射细胞中，这表明孔径足够大以将治疗剂递送到细胞中。这些发现有助于开发一种有效和安全的光疗和药物输送。
Abstract. The parameters critically influencing the delivery rate on the biodegradable microsphere-mediated femtosecond (fs) laser perforation are investigated in detail with the aim of developing efficient molecular delivery. Cell membrane was perforated by the irradiation of weakly focused fs laser pulses to the spherical polylactic acid microspheres conjugated to the cell membrane. The delivery of fluorescein isothiocyanate-dextran and fluorescent silica particles to A431 cells is investigated in detail. The increase in the number of irradiated laser pulses had resulted in the increase of delivery rate. The delivery rate depends on the size and functionalization of fluorescent silica particles in which silica particles of 100 nm in diameter were able to be delivered into 20% of the irradiated cells, suggesting that the pore sizes are large enough for the delivery of therapeutic agents into cells. These findings contribute to the development of an efficient and safe phototherapy and drug delivery.