Evidence that Candida albicans binds via a unique adhesion system on phagocytic cells in the marginal zone of the mouse spleen.
Evidence that Candida albicans binds via a unique adhesion system on phagocytic cells in the marginal zone of the mouse spleen.
复制标题
有证据表明白色念珠菌通过独特的粘附系统与小鼠脾边缘区的吞噬细胞结合。
DOI：
10.1128/iai.60.5.1972-1978.1992
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发表时间：
1992
影响因子：
3.1
通讯作者：
Cutler,JE
中科院分区：
文献类型：
--
作者：
Kanbe,T;Jutila,MA;Cutler,JE
我们最近通过体外粘附试验证明，白色念珠菌酵母细胞对脾脏边缘区表现出独特的结合亲和力。这种结合事件为研究真菌从血液中的器官传播机制提供了一个工作模型。通过使用离体检测报告在这里，我们表明，通过明场和电子显微镜技术，小鼠脾边缘区细胞能够ingredient印度墨水颗粒也参与酵母细胞附着。在脾脏清除体内循环中酵母细胞的过程中，C.白色念珠菌也仅与能够摄取印度墨水的边缘区细胞有关。摄取墨水颗粒的能力不一定与酵母细胞粘附有关，因为真菌细胞不与脾红髓中的吞噬细胞结合。事实上，边缘区吞噬细胞似乎有一个独特的结合系统，因为酵母细胞也不结合其他组织中的吞噬细胞，如胸腺和腹膜，或7种不同的骨髓细胞系。此外，一些充分表征的鼠粘附分子，如白细胞整合素，LECAM-1和CD 44的抗体，对结合没有影响。在这些结果的基础上，我们提出，脾边缘区吞噬细胞表达一种新的粘附系统，涉及一种独特的粘附分子或先前描述的粘附分子具有独特的结合活性。
We recently demonstrated by using an ex vivo adhesion assay that Candida albicans yeast cells exhibit a unique binding affinity for the marginal zone of the spleen. This binding event provides a working model for studying mechanisms of organ dissemination of the fungus from the blood. By using the ex vivo assays reported here, we showed by bright-field and electron microscopic techniques that mouse spleen marginal zone cells capable of ingesting India ink particles are also involved in yeast cell attachment. During splenic clearance of yeast cells from the circulation in vivo, C. albicans is also associated exclusively with marginal zone cells capable of ingesting India ink. The ability to ingest the ink particles is not necessarily related to yeast cell adherence, because the fungal cells did not bind to phagocytic cells in the splenic red pulp. In fact, the marginal zone phagocytic cells appear to have a unique binding system, because yeast cells also did not bind to phagocytes in other tissues, such as the thymus and peritoneum, or to seven different myeloid cell lines. In addition, antibodies to a number of well-characterized murine adhesion molecules, such as leukocyte integrins, LECAM-1, and CD44, had no effect on binding. On the basis of these results, we propose that splenic marginal zone phagocytes express a novel adhesion system that involves either a unique adhesion molecule or previously described adhesion molecules with unique binding activities.