Fibroblast growth factor receptor-1 expression is required for hematopoietic but not endothelial cell development
Fibroblast growth factor receptor-1 expression is required for hematopoietic but not endothelial cell development
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DOI：
10.1161/01.atv.0000163182.73190.f9
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发表时间：
2005-05-01
影响因子：
8.7
通讯作者：
Claesson-Welsh, L
中科院分区：
文献类型：
--
作者：
Magnusson, PU;Ronca, R;Claesson-Welsh, L
Objective-The purpose of this study was to clarify the role of fibroblast growth factors (FGFs) and FGF receptors (FGFRs) in hematopoietic/endothelial development.Methods and Results-Using several different FGFR-1-specific antibodies and FGFR-1 promoter-driven LacZ activity, we show that FGFR-1 is expressed and active as a tyrosine kinase in a subpopulation of endothelial cells (approximate to 20% of the endothelial pool) during development in embryoid bodies. In agreement, in stem cell-derived teratomas, expression of FGFR-1 was detected in some but not all vessels. The FGFR-1 expressing endothelial cells were mitogenically active in the absence and presence of vascular endothelial growth factor ( VEGF). Expression of FGFR-1 in endothelial cell precursors was not required for vascular development, and vascularization was enhanced in FGFR-1-deficient embryoid bodies compared with wild-type stem cells. In contrast, hematopoietic development was severely disturbed, with reduced expression of markers for primitive and definitive hematopoiesis.Conclusions-Our data show that FGFR-1 is expressed in early hematopoietic/endothelial precursor cells, as well as in a subpool of endothelial cells in tumor vessels, and that it is critical for hematopoietic but not for vascular development.
