Immunohistochemical detection of autotaxin (ATX)/lysophospholipase D (lysoPLD) in submucosal invasive colorectal cancer.
Immunohistochemical detection of autotaxin (ATX)/lysophospholipase D (lysoPLD) in submucosal invasive colorectal cancer.
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DOI：
10.1007/s12029-010-9186-4
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发表时间：
2011-12-01
影响因子：
1.6
通讯作者：
Nagawa, Hirokazu
中科院分区：
文献类型：
--
作者：
Kazama, Shinsuke;Kitayama, Joji;Nagawa, Hirokazu
目得：自分泌运动因子（ATX）是分子上相同的溶血磷脂酶D（lysoPLD），是一种主要的酶产生溶血磷脂酸（LPA），介导了广泛的细胞反应，包括刺激细胞motility.Patients和方法：使用免疫组化染色，我们研究了ATX/lysoPLD的表达在98例早期大肠癌粘膜下浸润。ATX/lysoPLD在肿瘤组织中的粘膜下层浸润细胞中高度表达，其特征为肥大细胞。结果：ATX/lysoPLD阳性细胞的数量在具有肉眼可见的凹陷病变的肿瘤中显著多于无凹陷病变的肿瘤。ATX/lysoPLD阳性细胞密度与微血管密度（MVD）呈正相关，而与淋巴管浸润、淋巴结转移及淋巴管密度（LVD）无相关性。结论：在大肠癌早期，ATX/lysoPLD局部产生LPA可能与抑制性病变的形成及肿瘤血管生成相关性较弱。
PURPOSE: Autotaxin (ATX) is molecularly identical to lysophospholipase D (lysoPLD) and is a main enzyme producing lysophosphatidic acid (LPA), which mediates a broad range of cellular responses including stimulation of cell motility.PATIENTS AND METHODS: Using immunohistochemical staining, we examined the expression of ATX/lysoPLD in 98 cases of early colorectal cancer with submucosal invasion. ATX/lysoPLD was highly expressed in infiltrating cells in tumor tissue in the submucosal layer, which were characterized as mast cells.RESULTS: The number of ATX/lysoPLD-positive cells was significantly greater in tumors with a macroscopically depressed lesion than in tumors without depression. The density of ATX/lysoPLD-positive cells tended to have a positive correlation with microvessel vascular density (MVD), while it was not correlated with vessel invasion and nodal metastases as well as lymphovascular vessel density (LVD).CONCLUSION: Our results suggest that local production of LPA through ATX/lysoPLD may weakly correlate with formation of a depressive lesion and tumor angiogenesis in the early stage of colorectal cancer.