Y-CHROMOSOME LOSS IN ESOPHAGEAL-CARCINOMA - AN IN-SITU HYBRIDIZATION STUDY
Y-CHROMOSOME LOSS IN ESOPHAGEAL-CARCINOMA - AN IN-SITU HYBRIDIZATION STUDY
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DOI：
10.1002/gcc.2870080306
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发表时间：
1993-11-01
影响因子：
3.7
通讯作者：
VARMA, V
中科院分区：
文献类型：
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作者：
HUNTER, S;GRAMLICH, T;VARMA, V
食道癌表现出强烈的男性优势，以及与其他部位发生的癌症的其他流行病学差异。在这项研究中，用福尔马林固定的石蜡包埋组织切片原位杂交的方法，评估了29例食道癌和53例发生在其他部位的食道癌的Y染色体缺失的发生率。Y染色体缺失的定义是：(1)肿瘤细胞Y染色阴性，而邻近的非肿瘤上皮和间质细胞Y染色阳性，(2)X和17号染色体的对照探针对肿瘤细胞染色阳性，(3)在不同的严格程度和蛋白质消化水平下，结果相似。根据这些标准，在14例食管腺癌中有13例(93%)存在Y染色体缺失，在13例食管鳞癌中有8例(62%)存在Y染色体缺失，在53例其他部位的癌中有5例(9%)发生Y染色体缺失。对于被检查的肿瘤，Y染色体缺失与食道癌，特别是腺癌有强烈和选择性的相关性(P<；.0001)。这些发现表明，Y染色体丢失可能在这些肿瘤中具有重要的病理意义。(C)1993年Wiley-Liss，Inc.*
Carcinoma of the esophagus shows a strong male predominance and other epidemiologic differences from cancers arising at other sites. In this study, the prevalence of Y chromosome loss in 29 carcinomas of the esophagus and 53 carcinomas arising elsewhere in the aerodigestive tract was assessed by in situ hybridization of formalin-fixed paraffin-embedded tissue sections. Absence of the Y chromosome was defined as (1) negative staining for Y in neoplastic cells with positive staining for Y in immediately adjacent nonneoplastic epithelial and stromal cells, (2) positive staining of neoplastic cells with control probes for chromosomes X and 17, and (3) similar results at different stringencies and levels of protein digestion. According to these criteria, absence of the Y chromosome was observed in 13 of 14 (93%) adenocarcinomas of the esophagus, 8 of 13 (62%) squamous cell carcinomas of the esophagus, and 5 of 53 (9%) carcinomas arising in other sites. For the neoplasms examined, Y chromosome deletion was strongly and selectively associated with carcinomas, particularly adenocarcinomas, of the esophagus (P < .0001). These findings suggest that Y chromosome loss may be pathogenetically significant in these neoplasms. (C) 1993 Wiley-Liss, Inc.*