Immunoneutralization of gonadotropin-releasing hormone type-III suppresses male reproductive behavior of cichlids
Immunoneutralization of gonadotropin-releasing hormone type-III suppresses male reproductive behavior of cichlids
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DOI：
10.1016/j.neulet.2006.02.041
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发表时间：
2006-08-07
影响因子：
2.5
通讯作者：
Parhar, Ishwar S.
中科院分区：
文献类型：
--
作者：
Ogawa, Satoshi;Akiyama, Go;Parhar, Ishwar S.
为探讨促性腺激素释放激素（GnRH）类型在生殖行为中的作用，将抗GnRH 1、GnRH 2和GnRH 3的抗血清立体定向注入雄性尼罗罗非鱼脑室内，以中和脑内3种天然GnRH类型。用免疫细胞化学方法观察了三种GnRH系统的形态学变化和繁殖行为（筑巢行为和攻击行为）。注射GnRH抗血清后，GnRH I、GnRH 2和GnRH 3免疫反应性纤维显著减少，表明成功中和了各自的内源性GnRH肽。GnRH1和GnRH2免疫中和不抑制繁殖行为，但GnRH3免疫中和显著降低筑巢能力（生理盐水：26.5 +/-3.7%/天对比GnRH3：6.1 +/-2.9%/天，P <0.001），巢大小（生理盐水：0.67 +/-0.09分vs GnRH3：0.10 +/-0.05分，P <0.0002）和攻击行为（生理盐水：2.34 +/-0.19分vs GnRH3 1.06 +/-0.12分，P <0.0001）。这些观察结果提供了证据，GnRH3是一种有效的神经调节剂的生殖行为在雄性罗非鱼。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
To investigate the roles of gonadotropin-releasing hormone (GnRH) types in reproductive behaviors, antisera against GnRH1, GnRH2 and GnRH3 were stereotaxically administered into the intracerebroventricular region to neutralize the three native GnRH types in the brain of male tilapia Oreochromis niloticus. Reproductive behaviors (nest-building and aggressive behaviors), and morphological changes of the three GnRH systems were investigated by immunocytochemistry. GnRH I, GnRH2 and GnRH3 immunoreactive fibers were significantly decreased following injections of GnRH antisera indicating successful neutralization of their respective endogenous GnRH peptides. GnRH1, and GnRH2-immunoneutralization did not inhibit reproductive behaviors but GnRH3-immunoneutralization significantly decreased nest-building ability (Saline: 26.5 +/- 3.7%/day versus GnRH3: 6.1 +/- 2.9%/day, P < 0.001), nest size (Saline: 0.67 +/- 0.09 points versus GnRH3: 0.10 +/- 0.05 points, P < 0.0002) and aggressive behavior (Saline: 2.34 +/- 0.19 points versus GnRH3 1.06 +/- 0.12 points, P < 0.0001). These observations provide evidence that GnRH3 is a potent neuromodulator of reproductive behaviors in male tilapia. (c) 2006 Elsevier Ireland Ltd. All rights reserved.