A simple method for short-term controlled anesthesia in newborn mice
A simple method for short-term controlled anesthesia in newborn mice
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DOI：
10.1016/j.physbeh.2004.03.013
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发表时间：
2004-09-15
影响因子：
2.9
通讯作者：
Gallego, J
中科院分区：
文献类型：
--
作者：
Drobac, E;Durand, E;Gallego, J
在这项研究中，我们描述了一种简单而廉价的方法，用于诱导新生小鼠的短期麻醉和快速恢复。在出生后第2、5和8天随机分配瑞士小鼠幼仔窝进行试验。通过将幼仔置于注射器中并加入一定体积的异氟烷饱和气体（产生估计水平为32%的异氟烷）诱导麻醉。异氟烷暴露持续30 s。所有的幼崽都在麻醉后存活了下来。在所有研究年龄，这种方法消除了对夹尾的伤害性反应，而不诱导死亡，因此显示出有效的麻醉。在异氟烷暴露后立即评估麻醉恢复，基于两个无害行为测试：对一滴水的防御反应（10次测试，间隔1分钟）和10分钟后的翻正反射，即，恢复俯卧位的时间（5次测试，间隔10分钟）。在恢复期，所有年龄组的水滴试验评分均向对照值增加。治疗和时间对翻正反射评分无显著影响。注射器中的初始体积、添加的异氟烷饱和气体的体积和暴露的持续时间可以根据出生后年龄和特定品系或物种（例如，大鼠）。这种方法非常适合发育中小鼠的行为或生理表型研究，其中有害程序必须在功能测试之前进行，使得从麻醉中快速恢复成为关键要求。(C)2004年爱思唯尔公司All rights reserved.
In this study, we describe a simple and inexpensive method for inducing short-term anesthesia and rapid recovery in newborn mice. Litters of Swiss mice pups were randomly allocated to testing on postnatal days 2, 5, and 8. Anesthesia was induced by placing the pup in a syringe and adding a volume of isoflurane-saturated gas that produced an estimated level of 32% isoflurane. Exposure to isoflurane lasted 30 s. All the pups survived the anesthesia. At all study ages, this method abolished the nociceptive response to tail clamp without inducing mortality, thus showing effective anesthesia. Recovery from anesthesia was assessed immediately after isoflurane exposure, based on two nonnoxious behavioral tests: the defensive response to a drop of water (10 tests, 1 min apart) and 10 min later the righting reflex, i.e., the time to recovery of the prone position (five tests, 10 min apart). The water drop test scores increased during the recovery phase toward the control values in all age groups. Treatment and time had no significant effect on righting reflex scores. The initial volume in the syringe, the volume of added isoflurane-saturated gas, and the duration of exposure may be adjusted according to postnatal age and specific strains or species (e.g., rats). This method is well suited to behavioral or physiological phenotype studies in developing mice, in which noxious procedures must precede functional testing, making rapid recovery from anesthesia a key requirement. (C) 2004 Elsevier Inc. All rights reserved.