Impact of various exercise modalities on hepatic mitochondrial function.
Impact of various exercise modalities on hepatic mitochondrial function.
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DOI：
10.1249/mss.0000000000000223
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发表时间：
2014-06
影响因子：
4.1
通讯作者：
Rector RS
中科院分区：
文献类型：
--
作者：
Fletcher JA;Meers GM;Linden MA;Kearney ML;Morris EM;Thyfault JP;Rector RS
肝脏线粒体对运动的适应性在很大程度上是未知的。在这里，我们试图确定不同运动方式对肝脏线粒体功能和新陈代谢测量的影响。将雄性SD大鼠随机分为安静(SED)、自愿轮跑(VWR)、暗周期拉食(VMR-OF)、跑台耐力训练(TM-End；30m/min，12%坡度，60min/d，5d/wk)和跑台间歇短跑训练(TM-IST；50m/min，12%坡度，6×2.5min，5d/wk)组，每组8~10只，进行4周干预。与SED模型组相比，4个运动组大鼠肝脏线粒体状态3和最大解偶联呼吸均显著增加(p<；0.05)。此外，VWR-OF、TM-END和TM-IST大鼠肝脏线粒体[1-14C]丙酮酸氧化为CO2，丙酮酸脱氢酶(PDH)活性指标显著增加(p<；0.05)，而运动诱导的[2-14C]丙酮酸氧化和[1-14C]棕榈酸氧化为CO2的增加没有统计学意义。肝线粒体sirtuin 3(SIRT3)蛋白含量在VWR、VWR-OF和TM-END组升高(p<；0.05)，推测SIRT3蛋白可增加线粒体蛋白的活性。此外，VWR-OF组和TM-END组大鼠肝脏细胞色素c蛋白和PEPCK m RNA含量增加，而PGC-1 CREB m RNA表达和磷酸化α蛋白含量增加。四周的运动训练，无论是什么运动方式，都显著增加了肝脏线粒体的呼吸，并引发了线粒体新陈代谢的其他独特改善，似乎不依赖于线粒体含量的增加。
Hepatic mitochondrial adaptations to exercise are largely unknown. Here we sought to determine the effects of various exercise modalities on measures of hepatic mitochondrial function and metabolism. Male Sprague Dawley rats were randomly assigned (n=8-10 per group) into sedentary (SED), voluntary wheel running (VWR), VWR with food pulled during the dark cycle (VMR-OF), treadmill endurance exercise (TM-END; 30 m/min, 12% gradient, 60 min/d, 5 d/wk), or treadmill interval sprint training (TM-IST; 50 m/min, 12% gradient, 6×2.5 min bouts, 5 d/wk) groups for a 4 week intervention. Hepatic mitochondrial state 3 and maximal uncoupled respiration were significantly (p<0.05) increased in all 4 exercise groups compared with SED animals. In addition, hepatic mitochondrial [1-14C] pyruvate oxidation to CO2, an index of pyruvate dehydrogenase (PDH) activity was significantly increased in VWR-OF, TM-END, and TM-IST rats (p<0.05); whereas, exercise-induced increases in [2-14C] pyruvate oxidation and [1-14C] palmitate oxidation to CO2 did not reach statistical significance. Hepatic mitochondrial sirtuin 3 (SIRT3) protein content, which putatively increases activity of mitochondrial proteins, was elevated in the VWR, VWR-OF and TM-END groups (p<0.05). Additionally, only VWR-OF animals experienced increases in hepatic cytochrome c protein content and PEPCK mRNA, while PGC-1α mRNA expression and phospho-CREB protein content was increased in VWR-OF and TM-END groups. Four weeks of exercise training, regardless of exercise modality, significantly increased hepatic mitochondrial respiration and evoked other unique improvements in mitochondrial metabolism that do not appear to be dependent on increases in mitochondrial content.