Thiobarbituric Acid Spray Reagent for Deoxy Sugars and Sialic Acids
Thiobarbituric Acid Spray Reagent for Deoxy Sugars and Sialic Acids
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DOI：
10.1038/186237a0
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发表时间：
1960-04
期刊：
影响因子：
64.8
通讯作者：
L. Warren
中科院分区：
文献类型：
--
作者：
L. Warren
最近，新的脱氧糖1,2 -酮，3-脱氧糖酸2,4和唾液酸5,6的灵敏检测方法被报道。在这些实验中，高碘酸盐氧化的产物，脱氧糖的丙二醛和后两组化合物的β-甲酰基丙酮酸，与2-硫代巴比妥酸偶联产生鲜红色的发色团。我想报告一种适用于喷纸色谱的方法。
RECENTLY, new sensitive assays have been reported for deoxy sugars1, 2-keto,3-deoxy sugar acids2–4, and sialic acids5,6. In these assays, the products of periodate oxidation, malonaldehyde from deoxy sugars and β-formylpyruvic acid from the latter two groups of compounds, are coupled with 2-thiobarbituric acid to produce a bright red chromophore. I wish to report an adaptation of these methods for spraying paper chromatograms.