Integron-containing bacteria in faeces of cattle from different production systems at slaughter
Integron-containing bacteria in faeces of cattle from different production systems at slaughter
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DOI：
10.1111/j.1365-2672.2009.04240.x
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发表时间：
2009-08-01
影响因子：
4
通讯作者：
Gobius, K. S.
中科院分区：
文献类型：
--
作者：
Barlow, R. S.;Fegan, N.;Gobius, K. S.
目的：确定草饲牛、批量饲喂牛或有机生产牛的粪便中含有整合子的细菌的流行率和特征。方法和结果：通过使用 PCR 和菌落杂交，检测草饲牛 (n = 125)、批量饲喂牛 (n = 125) 和有机牛 (n = 135) 的粪便样本中是否存在 1 类和 2 类整合子。批量饲养的牛（71% 和 62%）中 1 类和 2 类整合酶的患病率高于草饲牛（52% 和 30%），而草饲牛又高于有机牛（25% 和 11%）。含有整合子的细菌的分离率反映了 PCR 患病率结果。结论：整合子所携带的抗菌药物耐药性基因在三个系统中几乎没有差异，并且通常是很少用于治疗或促进生长目的的抗菌药物。系统之间观察到的患病率差异可能是每个系统强度的函数。该研究的意义和影响：无论抗菌药物的使用量如何，含整合子的细菌都可能存在于所有牛生产系统中，并证实需要谨慎使用抗菌药物，以避免产生含有对人类医学重要的基因的整合子。
Aims: To determine the prevalence and characteristics of integron-containing bacteria in faeces of cattle from grass-fed, lot-fed, or organically produced cattle.Methods and Results: Faecal samples from grass-fed (n = 125), lot-fed (n = 125) and organic (n = 135) cattle were tested for the presence of class 1 and class 2 integrons by using PCR and colony hybridisation. The prevalence of class 1 and class 2 integrase were higher in lot-fed cattle (71% and 62%) than grass-fed cattle (52% and 30%) which in turn were higher than organic cattle (25% and 11%). Isolation rates of integron-containing bacteria were reflective of PCR prevalence results.Conclusions: The antimicrobial resistance genes harboured by the integrons differed little across the three systems and were typically to antimicrobials that would rarely be used therapeutically or for growth promotion purposes. The differences in prevalence observed between the systems may be a function of the intensiveness of each system.Significance and Impact of the Study: Integron-containing bacteria may be present in all cattle production systems regardless of the amount of antimicrobial use and confirms that the prudent use of antimicrobials is required so that the development of integrons harbouring genes significant to human medicine is avoided.