BIOLOGY OF HUMAN MALIGNANT LYMPHOMAS .3. INTRACRANIAL HETEROTRANSPLANTATION IN NUDE, ATHYMIC MOUSE
BIOLOGY OF HUMAN MALIGNANT LYMPHOMAS .3. INTRACRANIAL HETEROTRANSPLANTATION IN NUDE, ATHYMIC MOUSE
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DOI：
10.1002/1097-0142(197605)37:5
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发表时间：
1976-01-01
期刊：
影响因子：
6.2
通讯作者：
KAPLAN, HS
中科院分区：
文献类型：
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作者：
EPSTEIN, AL;HERMAN, MM;KAPLAN, HS
无胸腺裸鼠颅内异种移植是人类恶性淋巴瘤实验生长的有效方法。 11 个原发性淋巴瘤和 6 个衍生细胞系的移植获得了高移植率 (90%) 和低平均潜伏期 (36 天)。相对较小的接种物产生了广泛的脑内浸润，可以通过免疫荧光将其鉴定为人类来源。尽管局限于中枢神经系统和脑膜，但肿瘤具有高度侵袭性，并且表现出与原始原发肿瘤惊人相似的形态特征。将淋巴瘤异种移植到颅外部位的情况很少成功。先前移植同源新生胸腺的裸鼠未能发展成脑内肿瘤。从几个颅内异种移植物中成功建立的次生细胞培养物被 NIH C 型异种病毒感染。描述了异种移植物的独特生长模式和其他神经病理学特征，并指出了这些观察结果与免疫抑制器官移植受者脑内淋巴瘤发展的相关性。这种体内研究人类恶性淋巴瘤的方法可以更好地了解肿瘤的组织病理学特征，并可以作为进一步实验研究的基础，包括病毒、免疫学和治疗研究。
Intracranial heterotransplantation in the nude, athymic mouse was an effective method for the experimental growth of human malignant lymphomas. Transplants of 11 primary lymphomas and 6 derived cell lines yielded a high take rate (90%) and a low mean latent period (36 days). Relatively small inocula produced extensive intracerebral infiltrates which could be identified as human in origin by immunofluorescence. Although confined to the CNS and meninges, the tumors were highly invasive and displayed morphologic features strikingly similar to those of the original primary tumors. Heterotransplantation of the lymphomas to extracranial sites was only rarely successful. Nude mice previously grafted with isologous neonatal thymuses failed to develop intracerebral tumors. Secondary cell cultures successfully established from several of the intracranial heterotransplants were infected with NIH type-C xenotropic virus. The distinctive growth patterns and other neuropathologic features of the heterotransplants are described, and the relevance of these observations to the development of intracerebral lymphomas in immunosuppressed organ transplant recipients is noted. This method of studying human malignant lymphomas in vivo may permit better histopathologic characterization of the tumors, and may serve as a basis for further experimental lines of investigation, including viral, immunologic and therapeutic studies.